Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF

When Recombinant Human TGF- beta 1 (Catalog # 240-B) is usedat 2 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT‑like 2 (Catalog # 10156-VN) bindswith an ED50 of 0.6‑3.6 μg/mL.

2 μg/lane of Recombinant Human Vasorin/SLIT-like 2 His-tag(Catalog # 10156-VN) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 83-95 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human TGF‑ beta 1 (Catalog # 240-B) is used at 2 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT‑like 2 (Catalog # 10156-VN) binds with an ED₅₀ of 0.6-3.6 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Vasorin/SLIT-like 2 protein
  Cys24-Asn573, with a C-terminal 6-His tag
• Accession #: Q6EMK4
• N-terminal Sequence: Cys24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 83-95 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF

• Atia
• Protein slit-like 2
• SLIT like 2
• Slitl2
• slit-like 2 (Drosophila)
• slit-like 2
• VASN
• Vasorin

Background

Vasorin, also known as SLIT-like 2, is an approximately 110-kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95-kDa soluble form of Vasorin can be shed from the cell surface by ADAM17-mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta-induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature human Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type III domain, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (1). Within the ECD, human Vasorin shares 85% and 86% aa sequence identity with mouse and rat Vasorin, respectively.

1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
2. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
3. Malapeira, J. et al. (2011) Oncogene 30:1912.
4. Li, S. et al. (2015) Oncotarget 6:10045.

Bioinformatics

• Uniprot:
  • Q6EMK4()