Recombinant Human ULBP-4/RAET1E Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ULBP-4/RAET1E Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to induce IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED 50 for this effect is typically 2-10 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human ULBP-4/RAET1E protein

  Human ULBP-4 (Gly30-Asp225) Accession # Q8TD07

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: Q8TD07
• N-terminal Sequence: Gly30
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: RAET1E
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ULBP-4/RAET1E Fc Chimera Protein, CF

• bA350J20.7
• LETAL
• LETALRAE-1-like transcript 4
• Lymphocyte effector toxicity activation ligand
• MGC125309
• N2DL4
• N2DL-4
• NKG2D ligand 4
• NKG2DL4
• RAET1E
• RAET1E2
• retinoic acid early transcript 1ERL-4
• RL-4
• ULBP4
• ULBP-4
• ULBP4MGC125308

Background

RAET1E (retinoic acid early transcript 1E), also called ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the RAET1/ULBP family of cell surface proteins that function as ligands for NKG2D (1-6). While most family members are GPI-anchored, only RAET1E/ULBP-4 and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human RAET1E mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, RAET1E shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha -epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of RAET1E mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down-regulated by retinoic acid (3). RAET1E is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). RAET1E binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma δ T cells, and CD8 + alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma δ T cells, direct RAET1E binding to both TCR gamma δ and NKG2D has been demonstrated (6). RAET1E is also thought to function as a minor histocompatibility antigen in humans (1).

1. Radosavljevic, M. et al. (2002) Genomics 79:114.
2. Kondo, M. et al. (2010) Immunogenetics 62:441.
3. Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
4. Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
5. Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
6. Kong, Y. et al. (2009) Blood 114:310.
7. Bacon, L. et al. (2004) J. Immunol. 173:1078.
8. Cao, W. et al. (2008) Int. Immunol. 20:981.

Bioinformatics

• Gene Symbol: RAET1E
• Uniprot:
  • Q8TD07(Human)