Recombinant Human SULT1B1 Protein, CF

Images

 
There are currently no images for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human SULT1B1 Protein, CF Summary

Details of Functionality
Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is >30 pmol/min/μg, as measured under the described conditions.
Source
E. coli-derived human Cytosolic Sulfotransferase 1B1/SULT1B1 protein
Leu2-Ile296, with an N-terminal 6-His tag
Accession # O43704
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Enzymes
Gene
SULT1B1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publication using
5959-ST in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 50 mM Tris, 15 mM MgCl2, pH 7.5 (supplied in 10X form in kit)
  • Recombinant Human Cytosolic Sulfotransferase 1B1/SULT1B1 (rhSULT1B1) (Catalog # 5959-ST)
  • 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) (Catalog # ES019)
  • 1-Naphthol (Sigma, Catalog # N1000), 10 mM in deionized water
  • Universal Sulfotransferase Activity Kit (Catalog # EA003)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute 1 mM Phosphate Standard provided by the Universal Sulfotransferase Kit by adding 40 µL of the 1 mM Phosphate Standard to 360 µL of Assay Buffer for a 100 µM stock. This is the first point of the standard curve.
  2. Prepare additional curve points by performing six one-half serial dilutions of the 100 µM Phosphate stock in Assay Buffer. The standard curve has a range of 0.078 to 5 nmol per well.
  3. Prepare reaction mixture containing 0.4 mM PAPS, 0.4 mM 1-Naphthol, and 0.02 mg/mL Coupling Phosphatase 3 in Assay Buffer.
  4. Dilute rhSULT1B1 to 40 µg/mL in Assay Buffer.
  5. Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Assay Buffer.
  6. Load 25 µL of the 40 µg/mL rhSULT1B1 into the plate. Include 25 µL of Assay Buffer to be used as a Control.
  7. Add 25 µL of reaction mixture to the wells, excluding the standard curve.
  8. Cover the plate with a plate sealer and incubate at 37 °C for 20 minutes.
  9. Add 30 µL of the Malachite Green Reagent A to all wells. Mix briefly.
  10. Add 100 µL of deionized water to all wells. Mix briefly.
  11. Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
  12. Read plate at 620 nm (absorbance) in endpoint mode.
  13. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Phosphate released* (nmol) x (1000 pmol/nmol)
Incubation time (min) x amount of enzyme (µg)

     *Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control.

Per Reaction:
  • rhSULT1B1: 1.0 µg
  • PAPS: 10000 pmol (0.2 mM)
  • 1-Naphthol: 0.2 mM
  • Coupling Phosphatase 3: 0.5 µg

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SULT1B1 Protein, CF

  • Cytosolic Sulfotransferase 1B1
  • EC 2.8.2
  • EC 2.8.2.-
  • EC 2.8.2.1
  • EC 2.8.2.4
  • MGC13356
  • ST1B1
  • ST1B2
  • ST1B2sulfotransferase family cytosolic 1B member 1
  • Sulfotransferase 1B1
  • Sulfotransferase 1B2
  • sulfotransferase family, cytosolic, 1B, member 1
  • SULT1B1
  • SULT1B2
  • Thyroid hormone sulfotransferase

Background

Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1-3). They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols (4). Human SULT1B1 shows 72.3% amino acid sequence identity to mouse SULT1B1.The enzymatic activity of the recombinant human GAL3ST2 is measured using a phosphatase-coupled assay (4).

  1. Falany, C, N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Allali-Hassani, A. et al. (2007) PLoS Biol. 5:e97.
  4. Wang, J. et al. (1998) Mol. Pharmacol. 53:274.
  5. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Customers Who Viewed This Item Also Viewed...

NBP2-03227
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-00486
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-32604
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84786
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-11538
Species: Hu, Mu, Rt
Applications: WB, IHC-P
NBP2-57642
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-31904
Species: Hu
Applications: WB, IHC, IHC-P
AF6490
Species: Hu
Applications: WB, IHC, ICC
NBP1-81770
Species: Hu
Applications: WB, IHC, IHC-P
AF6174
Species: Hu
Applications: WB, IHC, IP
NBP2-49113
Species: Hu
Applications: IHC, IHC-P
NBP1-59786
Species: Hu
Applications: WB
NBP2-37502
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-02563
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-22124
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
5959-ST
Species: Hu
Applications: Enzyme Activity

Publications for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: EnzAct.


Filter By Application
EnzAct
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST) (0)

There are no reviews for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST)

Discover related pathways, diseases and genes to Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST)

Discover more about diseases related to Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST).
 

Pathways for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST)

View related products by pathway.

PTMs for Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST)

Learn more about PTMs related to Cytosolic Sulfotransferase 1B1/SULT1B1 (5959-ST).

Blogs on Cytosolic Sulfotransferase 1B1/SULT1B1

There are no specific blogs for Cytosolic Sulfotransferase 1B1/SULT1B1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human SULT1B1 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol SULT1B1
Uniprot