Recombinant Human SSc5D His-tag Protein, CF

Recombinant Human SSc5D His-tag (Catalog # 10535-SS) binds fluorescein-conjugated E. coli bioparticles. The ED50 for this effect is 0.2-1 μg/mL.

2 μg/lane of Recombinant Human SSc5D His-tag Protein (10535-SS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 120-155 at kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SSc5D His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED₅₀ for this effect is 0.2-1 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human SSc5D protein
  Val17-Thr875, with a C-terminal 6-His tag
• Accession #: NP_001138422.1
• N-terminal Sequence: Val17
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-153 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SSc5D His-tag Protein, CF

• S5D-SRCRB
• scavenger receptor cysteine rich domain containing (5 domains)
• scavenger receptor cysteine-rich glycoprotein
• soluble scavenger protein with 5 SRCR domains
• soluble scavenger receptor cysteine-rich domain-containing protein SSC5D
• soluble scavenger with 5 domains
• SSc5D

Background

SSc5D (Soluble Scavenger with 5 Domains) is a member of scavenger receptor cysteine-rich (SRCR) superfamily that comprises a group of proteins with one or more domains sharing structural homology with the membrane distal extracellular domain of the macrophage type I scavenger receptor (1). Mature human SSc5D consists of 1573 amino acids (aa), including five SRCR domains at the N-terminus and a heavily glycosylated, mucin-like domain at the C-terminus. Within N‑terminal SRCR domains, human SSc5D shares 95% and 96% aa sequence identity with mouse and rat SSc5D, respectively (1). SSc5D is produced by monocyte/macrophages, T cells and several epithelial cells from placenta, spleen, and colon (1). Many members of the SRCR super family bind to and clear bacteria, fungi or viruses from infected host (2). SSc5D physically interacts with whole bacteria cells especially to E. coli strains. These findings can help fight bacterial infections by discriminating between different bacteria strains and species (3).

1. Goncalves, C.M. et al. (2009) Mol. Immunol. 46:2585.
2. Martinez, V.G. et al. (2011) Pharmacol. Rev. 63:967.
3. Bessa Pereira, C. et al. (2016) Front. Immunol. 7:416.

Bioinformatics

• Uniprot:
  • NP_001138422.1()