Recombinant Human SMOC-2 Protein, CF

Images

 
There are currently no images for SMOC-2 (5140-SM).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human SMOC-2 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.

When 5 x 104 cells/well are added to rhSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is 0.4‑1.6 μg/mL.

Source
Chinese Hamster Ovary cell line, CHO-derived human SMOC-2 protein
Gln22-Gly446, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
SMOC2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
48.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
58-66 kDa, reducing conditions
Publications
Read Publications using
5140-SM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SMOC-2 Protein, CF

  • bA270C4A.1
  • bA37D8.1
  • dJ421D16.1
  • MST117
  • MSTP117
  • MSTP140
  • SMAP2
  • SMAP-2
  • SMAP2Secreted modular calcium-binding protein 2
  • SMOC2
  • SMOC-2
  • smooth muscle associated protein 2
  • Smooth muscle-associated protein 2
  • SPARC related modular calcium binding 2
  • SPARC-related modular calcium-binding protein 2
  • thyroglobulin type-1 repeat containing protein

Background

SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins (1, 2). The human SMOC-2 cDNA encodes 446 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40 - 84), two thyroglobulin type-1 segments (aa 87 - 153 and 213 - 281) and two EF-hand sequences (aa 347 - 382 and 384 - 419) (1, 2). Of three splice variants, one shows a 13 aa substitution for aa 442 - 446, another shows an 11 aa insertion after Thr170, and a third contains both of these variations. Mature human SMOC-2 shares 95%, 95% and 94% aa identity with mouse, rat and canine SMOC-2, respectively. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions (1 - 5). Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alpha v beta 1 and alpha v beta 6 (3, 6). SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1 (4). When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis (5). SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle (2). In the skin, it promotes keratinocyte attachment and migration (6). Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery (2, 7).

  1. Vannahme, C. et al. (2003) Biochem. J. 373:805.
  2. Nishimoto, S. et al. (2002) Biochim. Biophys. Acta 1576:225.
  3. Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
  4. Liu, P. et al. (2008) Mol. Biol. Cell 19:248.
  5. Rocnik, E.F. et al. (2006) J. Biol. Chem. 281:22855.
  6. Maier, S. et al. (2008) Exp. Cell Res. 314:2477.
  7. Wilk, J.B. et al. (2007) Am. J. Respir. Crit. Care Med. 175:554.

Customers Who Viewed This Item Also Viewed...

AF5550
Species: Mu, Rt
Applications: WB
H00003611-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, RNAi
AF942
Species: Mu
Applications: WB, IHC, CyTOF-ready, ICFlow
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-83702
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13506
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-79781
Species: Hu
Applications: WB, IHC, IHC-P, IP
AF5550
Species: Mu, Rt
Applications: WB
NBP2-61832
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-32895
Species: Hu, Mu, Rt
Applications: WB
NBP2-67861
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49643
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-IC, KO
NB100-56350
Species: Hu, Mu, Rt
Applications: WB
NBP1-81259
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
7584-SF/CF
Species: Hu
NBP2-41263
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
AF3688
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC

Publications for SMOC-2 (5140-SM)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Surface Plasmon Resonance.


Filter By Application
Bioassay
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for SMOC-2 (5140-SM) (0)

There are no reviews for SMOC-2 (5140-SM). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for SMOC-2 (5140-SM) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

TGN46 Antibody
NBP1-49643

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human SMOC-2 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol SMOC2
Entrez
Uniprot