Recombinant Human Semaphorin 4C Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Semaphorin 4C Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit survival of SK-OV-3 human ovarian carcinoma cells. The ED 50 for this effect is 1-5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Semaphorin 4C protein

  Human Semaphorin 4C (Ala21-Gly663) Accession # Q9C0C4

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: Q9C0C4
• N-terminal Sequence: Ala21
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SEMA4C
• Purity: >85%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 102-123 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citric acid, NaCl and Trehalose.
• Purity: >85%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 4C Fc Chimera Protein, CF

• FLJ20369
• KIAA1739
• MGC126382
• MGC126383
• M-Sema F
• M-SEMA-F
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C
• SEMA4C
• SEMACL1
• Semaf
• SEMAI
• Semaphorin 4C
• semaphorin-4C

Background

Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a member of the class IV semaphorin family of type 1 transmembrane glycoproteins (1). Semaphorin 4C signaling is involved in cell attraction and repulsion as well as neural tube closure, stem cell proliferation, and kidney development. Mature Semaphorin 4C consists of a 643 amino acid (aa) extracellular region, a 21 aa transmembrane domain, and a 149 aa PDZ-containing cytoplasmic tail (2-4). The extracellular domain of mature human Semaphorin 4C shares 85% and 84% aa sequence identity with mouse and rat Semaphorin 4C, respectively, and is characterized by a Sema domain, a cysteine rich PSI domain, and an Ig-like C2 -type domain. Semaphorin 4C is widely expressed during embryogenesis with high expression within the neural tube (5-7). Comparison of Plexin B2 -/- and Sema4C -/- mice revealed similar phenotypes, including neural tube closure defects and neonatal lethality. Viable Sema4C -/- neonates have an abnormally developed cerebellum and defects in ventral skin pigmentation (8). Semaphorin 4C has been shown to induce cerebellar granule cell precursor migration and neural stem cell proliferation in in vitro and ex vivo assays, respectively (9). Involvement of Semaphorin 4C in neural stem cell proliferation is further supported by the down-regulation of its expression in neuroblasts during differentiation and up-regulation in proliferating Nestin-postive cells following ischemia (2). Semaphorin 4C is a high affinity ligand for Plexin B2. Upon activation by Sema4C, Plexin B2 can phosphorylate Tyr 1248 on the receptor tyrosine kinase, ErbB2, which then activates RhoA to regulate cytoskeletal dynamics as well as neuronal migration and proliferation (9, 10). During embryogenesis, Semaphorin 4C-Plexin B2 signaling also stimulates branching of the ureteric epithelium in the kidney (11). In adult tissues, the expression pattern of Semaphorin 4C in non-neuronal tissues indicates that Semaphorin 4C-Plexin B2 signaling may additionally regulate vascular and endocrine function (12). Similar to Semaphorin 3A (13, 14), R&D Systems’ in-house testing data indicate that Semaphorin 4C can inhibit cell survival.

1. Tessier-Lavigne, M. and C.S. Goodman (1996) Science 274:1123.
2. Wu, H. et al. (2009) J. Mol. Neurosci. 39:27.
3. Wang, L. et al. (1999) J. Biol. Chem. 274:14137.
4. Inagaki, S. et al. (1995) FEBS Lett. 370:269.
5. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
6. Perala, N. et al. (2010) Dev. Dyn. 239:2722.
7. Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
8. Maier, V. et al. (2011) Mol. Cell. Neurosci. 46:419.
9. Deng, S. et al. (2007) J. Neurosci. 27:6333.
10. Swiercz, J.M. et al. (2004) J. Cell. Biol. 165:869.
11. Perala, N. et al. (2011) Differentiation 81:81.
12. Zielonka, M. et al. (2010) Exp. Cell Res. 316:2477.
13. Shirvan, A. et al. (1999) J. Neurochem. 73:961.
14. Guttman-Raviv, N. et al. (2007) J. Biol. Chem. 282:26294.

Publications for Semaphorin 4C (6125-S4)(1)

Publication using 6125-S4

• C Junqueira, R Dariolli, J Haydak, S Kang, T Hannah, RJ Wiener, S DeFronzo, R Tejero, GL Gusella, A Ramakrishn, R Alves Dias, A Wojcinski, S Kesari, L Shen, EA Sobie, JP Rodrigues, EU Azeloglu, H Zou, RH Friedel Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion Nature Communications, 2021-10-14;12(1):6019. 2021-10-14 [PMID: 34650052] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SEMA4C
• Uniprot:
  • Q9C0C4(Human)