Recombinant Human RGM-C/Hemojuvelin Protein, CF


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Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human RGM-C/Hemojuvelin Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rhBMP-4 at 1 µg/mL can bind rhRGM-C with a linear range of
10‑500 ng/mL.
Mouse myeloma cell line, NS0-derived human RGM-C/Hemojuvelin protein
Gln36-Asp400 (mature) & Pro173-Asp400 (C-terminus chain), both with a C-terminal 6-His tag & Gln36-Asp172 (N-terminus chain)
Accession #
N-terminal Sequence
Pro173 & No results obtained: Gln36 predicted
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
40 kDa (mature), 14 kDa (N-terminus chain), 26 kDa (C-terminus chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50-55 kDa, 20 kDa and 32-37 kDa, reducing conditions
Read Publications using
3720-RG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human RGM-C/Hemojuvelin Protein, CF

  • DL-M
  • haemojuvelin
  • hemochromatosis type 2 (juvenile)
  • Hemojuvelin
  • HFE2
  • HFE2AMGC23953
  • HJV
  • HJVHemochromatosis type 2 protein
  • JH
  • repulsive guidance molecule c
  • RGMC
  • RGM-C
  • RGMCRGM domain family member C


RGM-C, also known as hemojuvelin, is a member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane glycoproteins (1, 2). RGM-C is expressed in striated muscle and periportal hepatocytes (3 - 5). The protein undergoes partial cleavage intracellularly, resulting in a disulfide-linked dimer of the 14 kDa N-terminal and 33 kDa C-terminal portions (4, 6, 7). The N-terminal fragment contains an RGD motif, while the C-terminal fragment carries the GPI attachment site (4, 7). Two alternatively spliced isoforms lack either approximately half or the entire N-terminal fragment. Full length RGM-C can also be released from the cell and circulates in the blood (6, 8). RGM-C is disrupted in type 2A juvenile hemochromatosis, a hereditary iron homeostasis disorder characterized by excessive iron accumulation (5). In mouse, loss of RGM-C function results in decreased expression of the iron regulatory hormone hepicidin and increased iron deposition in liver, pancreas, and heart (5, 9). Membrane associated RGM-C upregulates hepicidin while soluble RGM-C downregulates hepicidin expression (8). This appears to be an iron-responsive regulatory system, as high blood iron levels reduce the amount of soluble RGM-C produced (8). RGM-C, similar to RGM-A, associates with neogenin (7). Disease-related point mutations can prevent internal RGM-C cleavage or its ability to interact with neogenin (6, 7). Experimental inflammatory conditions result in decreased RGM-C expression and increased hepicidin expression, although the two effects occur independently (5, 10). RGM-C also functions as a BMP coreceptor and enhances BMP-2 and BMP-4 signaling (11). In this context, RGM-C enhances the BMP-2 upregulation of hepatic hepicidin (11). Mature human RGM-C shares 89% amino acid (aa) sequence identity with mouse and rat RGM-C. It shares 49% and 44% aa sequence identity with human RGM-A and RGM-B, respectively.

  1. Papanikolaou, G. et al. (2004) Nat. Genet. 36:77.
  2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  3. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  5. Niederkofler, V. et al. (2005) J. Clin. Invest. 115:2180.
  6. Kuninger, D. et al. (2006) J. Cell Sci. 119:3273.
  7. Zhang, A.S. et al. (2005) J. Biol. Chem. 280:33885.
  8. Lin, L. et al. (2005) Blood 106:2884.
  9. Huang, F.W. et al. (2005) J. Clin. Invest. 115:2187.
  10. Krijt, J. et al. (2004) Blood 104:4308.
  11. Babitt, J.L. et al. (2006) Nat. Genet. 38:531.

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Publications for RGM-C/Hemojuvelin (3720-RG)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA development, Surface Plasmon Resonance.

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ELISA development
Surface Plasmon Resonance
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Gene Symbol HFE2