Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human RELT/TNFRSF19L protein
  

  Human RELT Ser26-Ala160 (Arg127Gly and Arg129Gly) Accession #Q969Z4	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q969Z4
• N-terminal Sequence: Ser26
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: RELT
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-58 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF

• Receptor expressed in lymphoid tissues
• RELT tumor necrosis factor receptor
• RELT
• TNFRSF19L
• TNFRSF19LFLJ14993
• tumor necrosis factor receptor superfamily member 19L
• tumor necrosis factor receptor superfamily, member 19-like

Background

RELT (Receptor Expressed in Lymphoid Tissues) is a 46 kDa (predicted) type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily, designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes (2, 3). Human RELT cDNA encodes 430 amino acids (aa), including a 25 aa signal peptide, a 137 aa extracellular domain containing a TNF receptor cysteine-rich domain and a potential N‑linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region that lacks a death domain. Within the ECD, human RELT shares 78%, 75%, 75%, 78% and 72% aa sequence homology with mouse, rat, canine, porcine and bovine RELT, respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TROY/TNFRSF19 and OX40 (2). Neither human nor mouse RELT bind any of the ~19 TNF superfamily ligands that have been tested (1). Two related transmembrane proteins, RELL1 and RELL2, have been identified in both human and mouse (3). RELL1 and 2 can interact with and are coexpressed with RELT, and are thought to modulate its signaling (3-5). Intracellularly, RELT has been shown to bind the adaptor protein TRAF-1 and activate the NF‑ kappa B pathway, the phospholipid scramblase PLSCR1, and oxidative stress responsive proteins OSR1 (2-5). Another investigator notes association and signaling through SPAK, but not TRAF or NF kappa B (6). When overexpressed in HEK-293 cells, RELT induces p38 and JNK signaling and activates apoptosis (4-6). Immobilized RELT can also costimulate T-cell proliferation in the presence of CD3 signaling (2).

1. Bossen C. et al. (2006) J. Biol. Chem. 281:13964.
2. Sica, G. et al. (2001) Blood 97:2702.
3. Cusick, J.K. et al. (2006) Biochem. Biophys. Res. Commun. 340:535.
4. Cusick, J.K. et al. (2010) Cell. Immunol. 261:1.
5. Cusick, J.K. et al. (2012) Mol. Cell. Biochem. 362:55.
6. Polek T.C. et al. (2006) Biochem. Biophys. Res. Commun. 343:125.

Bioinformatics

• Gene Symbol: RELT
• Uniprot:
  • Q969Z4()