Recombinant Human PTGES2 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human PTGES2 Protein, CF Summary

• Source: E. coli-derived human Prostaglandin E Synthase 2/PTGES2 protein
  Glu88-His377, with an N-terminal Met and 6-His tag
• Accession #: Q9H7Z7
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Enzymes
• Gene: PTGES2
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-32 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Notes

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PTGES2 Protein, CF

• C9orf15
• chromosome 9 open reading frame 15
• EC 5.3.99.3
• FLJ14038
• gamma-interferon-activated transcriptional element-binding factor 1
• GATE-binding factor 1
• GBF1
• membrane-associated prostaglandin E synthase 2
• Microsomal prostaglandin E synthase 2
• microsomal prostaglandin E synthase-2
• mPGES2
• mPGES-2
• PGES2
• PGES2MGC11289
• prostaglandin E synthase 2
• PTGES2

Background

PTGES2, also known as membrane-associated prostaglandin E synthase (mPGES) catalyzes the conversion of prostaglandin H₂ (PGH₂) to prostaglandin E₂ (PGE₂). Its N-terminal domain anchors it in the Golgi membrane, and its catalytic domain resides in the cytoplasm. Proteolysis within the cell generates a truncated soluble form beginning with amino acid 88 that resides in the cytosol (1). PTGES2 production of PGE₂ is coupled to both COX-1 and COX-2 (2). PTGES2 is expressed in many tissues, with highest expression in brain, heart, skeletal muscle, kidney, and liver (1). In addition to its PGE₂ production, PTGES2 activates transcription regulated by a gamma-interferon-activated transcription element (3). The protein may also play a role in colorectal cancer progression (4).

1. Tanikawa, N. et al. (2002) Biochem. Biophys. Res. Commun. 291:884.
2. Murakami, M. et al. (2003) J. Biol. Chem. 278:37937.
3. Hu, J. et al. (2002) J. Biol. Chem. 277:30253.
4. Seo, T. et al. (2009) Virchows Arch. 454:667.
   
   

Bioinformatics

• Gene Symbol: PTGES2
• Uniprot:
  • Q9H7Z7(Human)