Recombinant Human Protocadherin-18 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Protocadherin-18 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 0.04-0.2 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Protocadherin-18 protein
  Lys28-Ser699, with a C-terminal 6-­His tag
• Accession #: Q9HCL0
• N-terminal Sequence: Lys28
• Protein/Peptide Type: Recombinant Proteins
• Gene: PCDH18
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Protocadherin-18 Protein, CF

• KIAA1562
• PCDH68L1
• protocadherin 18
• Protocadherin18
• Protocadherin-18

Background

Protocadherin-18 (PCDH18) is an approximately 130 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins (1-3). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1, 2, 4). Within the ECD, human Protocadherin-18 shares 92% and 91% amino acid sequence identity with mouse and rat Protocadherin-18, respectively. Protocadherin-18 is expressed in the brain, heart, kidney, lung, and trachea (1, 5). Protocadherin-18 promotes cell-cell adhesion during embryogenesis in Zebrafish (6). Mouse Protocadherin-18 interacts with Disabled-1, a protein required for correct formation of cortical neuron layers (4, 5, 7, 8). Also in mice, Protocadherin-18 may additionally be an activation marker for CD8⁺ memorty T cells (9). In humans, deletion of Protocadherin-18 has been linked to intellectual disability (10).

1. Wolverton, T. and M. Lalande (2001) Genomics 76:66.
2. Vanhalst, K. et al. (2005) Cell. Mol. Life Sci. 62:1247.
3. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.
4. Redies, C. et al. (2005) Cell. Mol. Life Sci. 62:2840.
5. Homayouni, R. et al. (2001) Biochem. Biophys. Res. Commun. 289:539.
6. Aamar, E. and I.B. Dawid (2008) Dev. Biol. 318:335.
7. Howell, B.W. et al. (1999) Genes Dev. 13:643.
8. Howell, B.W. et al. (2000) Curr. Biol. 10:877.
9. Vazquez-Cintron, E.J. et al. (2012) PLoS One 7:e36101.
10. Kasnauskiene, J. et al. (2012) Eur. J. Med. Genet. 55:274.

Bioinformatics

• Gene Symbol: PCDH18
• Uniprot:
  • Q9HCL0()