Recombinant Human Protocadherin-17 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Protocadherin-17 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 0.06-0.24 ug/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Protocadherin-17 protein
  Leu18-Ser705, with a C-terminal 6-His tag
• Accession #: O14917
• N-terminal Sequence: Leu18
• Protein/Peptide Type: Recombinant Proteins
• Gene: PCDH17
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 75.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Protocadherin-17 Protein, CF

• PCDH17
• PCDH68
• PCDH68protocadherin-68
• PCH68
• PCH68Protocadherin-68
• protocadherin 17
• protocadherin 68
• Protocadherin17
• Protocadherin-17

Background

Human Protocadherin 17 (PCDH17; also called Protocadherin‑68) is an approximately 1159 amino acid (aa) glycoprotein belonging to the δ2 subgroup of protocadherins, type I transmembrane proteins that exhibit calcium‑dependent but relatively weak homophilic adhesion (1‑3). Like other δ2 protocadherins, PCDH17 cDNA encodes a signal sequence (aa 1‑17), six cadherin domains of about 100 aa each (aa 18‑695), a transmembrane sequence and a cytoplasmic domain (1). It contains an extracellular RGD cell attachment motif that may mediate integrin adhesion (3). Typical of protocadherins, an alternately spliced 889 aa form of human PCDH17 diverges at aa 876 within the cytoplasmic domain and lacks two conserved cytoplasmic motifs (1, 2). Within the extracellular domain, human PCDH17 shares 98% aa sequence identity with mouse and bovine, 99% with rat and equine, and 97% with porcine PCDH17. PCDH17 is constitutively expressed in the central nervous system (3). It is one of several PCDH molecules that shows region specificity in the basal ganglia, forming gradients (3). Early in postnatal life, it is preferentially expressed in motor and auditory cortices and is correlated with thalamo‑cortical and intrahippocampal circuits (3‑5). Its expression is increased in the Broadman area of the brain in subjects with schizophrenia (3). PCDH17 is frequently silenced in squamous cell carcinomas such as those in the esophagus and larynx, especially if they are poorly differentiated (3, 6, 7). Ectopic PCDH10 expression reduces tumor cell proliferation and migration (6).

1. Redies, C. et al. (2005) Cell. Mol. Life Sci. 62:2840.
2. Vanhalst, K. et al. (2005) Cell. Mol. Life Sci. 62:1247.
3. Kim, S. Y. et al. (2011) Cell Adh. Migr. 5:97.
4. Kim, S. Y. et al. (2007) Neuroscience 147:996.
5. Kim, S. Y. et al. (2010) Neuroscience 170:189.
6. Haruki, S. et al. (2010) Carcinogenesis 31:1027.
7. Giefing, M. et al. (2011) Genes Chromosomes Cancer 50:154.

Bioinformatics

• Gene Symbol: PCDH17
• Uniprot:
  • O14917(Human)