Recombinant Human Plexin B2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Plexin B2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 is coated at 5 μg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4) binds with an apparent K_d <5 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Plexin B2 protein
  Leu20-Arg1160, with a C-terminal 6-His tag
• Accession #: O15031
• N-terminal Sequence: Leu20
• Protein/Peptide Type: Recombinant Proteins
• Gene: PLXNB2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 125.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 145-180 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Plexin B2 Protein, CF

• MM1
• MM1KIAA0315dJ402G11.3
• Nbla00445
• PLEXB2
• Plexin B2
• plexin-B2
• PLXNB2

Background

Plexin B2 is a 240 kDa type I transmembrane (TM) glycoprotein of the Plexin B family of semaphorin receptors (1, 2). The human Plexin B2 cDNA encodes 1838 amino acids (aa) that include a 19 aa signal sequence, a 1178 aa extracellular domain (ECD), a 21 aa TM domain, and a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20-466) and three IPT repeats (aa 803-1092). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20-1164) and an 80 kDa TM beta ‑chain, that remain noncovalently‑linked (1). Multiple splice variants may exist. Within aa 20-1160 in the ECD, human Plexin B2 shares 82%, 83%, 82% and 81% aa identity with mouse, rat, canine and bovine Plexin B2, respectively. The human B Plexins (B1, B2 and B3) share 39-47% aa identity with each other. Plexin B2 mRNA is expressed in proliferating cerebellar granule cell progenitors, neuroepithelium, developing neurons, growth plate chondrocytes, tooth bud inner enamel epithelium, glomeruli and mesenchyme of the developing kidney, and in germinal center B lymphocytes when T cell help is present (3-7). Plexin B2 is often co‑expressed with Plexin B1, and the two may form heterodimers (1, 4, 6). Genetic deletion of mouse Plexin B2 results in defects in proliferation and migration of cerebellar granule cells, abnormal development of the neural tube and disorganization of the embryonic brain; these defects are not seen when Plexin B1 is deleted (8, 9). Plexin B2 serves as a receptor for type 4 semaphorins, especially Sema4C (8-10). B Plexins, including Plexin B2, can bind the scatter factor receptors, Met and Ron, and activate them upon semaphorin engagement (1, 11).

1. Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
2. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
3. Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
4. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
5. Zhang, M. et al. (2008) Bone 43:511.
6. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
7. Yu, D. et al. (2008) Immunol. Cell Biol. 86:3.
8. Deng, S. et al. (2007) J. Neurosci. 27:6333.
9. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
10. Yukawa, K. et al. (2010) Int. J. Mol. Med. 25:225.
11. Conrotto, P. et al. (2004) Oncogene 23:5131.

Publications for Plexin B2 (5329-PB)(1)

Publication using 5329-PB

• Borrelli, C;Roberts, M;Eletto, D;Hussherr, MD;Fazilaty, H;Valenta, T;Lafzi, A;Kretz, JA;Guido Vinzoni, E;Karakatsani, A;Adivarahan, S;Mannhart, A;Kimura, S;Meijs, A;Baccouche Mhamedi, F;Acar, IE;Handler, K;Ficht, X;Platt, RJ;Piscuoglio, S;Moor, AE; In vivo interaction screening reveals liver-derived constraints to metastasis Nature 2024-07-24 [PMID: 39048831] (Bioassay, Human)

Bioinformatics

• Gene Symbol: PLXNB2
• Uniprot:
  • O15031()