Recombinant Human Plexin A1 His-tag Protein, CF

Recombinant Human PLXNA-1/C-His (Catalog # 10167-PA) has a molecular weight (MW) of 156.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

2 μg/lane of Recombinant Human Plexin A1 His-tag (Catalog # 10167-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa...

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Plexin A1 His-tag Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin A1 His-tag is immobilized at 2.50 µg/mL (100 µL/well), Recombinant Human Semaphorin 6C Fc Chimera (Catalog # 2219-S6) binds with an ED₅₀ of 1.00-6.00 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Plexin A1 protein
  Glu27 - Pro1244, with a C-terminal 6-His tag
• Accession #: Q9UIW2.3
• N-terminal Sequence: Glu27
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-150 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Plexin A1 His-tag Protein, CF

• NOV
• NOVP
• Plexin A1
• PLEXIN-A1
• PLXN1
• PLXNA1

Background

Plexin A1, also known as Plexin 1, is a type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (2). Mature human Plexin A1 consists of an extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a transmembrane segment, and a large cytoplasmic domain. Within the ECD, human Plexin A1 shares 95% amino acid sequence identity with mouse and rat Plexin A1. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2 and binds transmembrane Semaphorin 6D directly (3 ‑ 5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5-8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12). Plexin A1 has also been identified as a receptor for Slits and mediate commissural growth cone collapse (13).

1. Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
2. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
3. Takamatsu, H. et al. (2010) Cell. Mol. Immunol. 7:83.
4. O’Connor, B.P. and J.P.Y. Ting (2008) Immunol. Res. 41:217.
5. Takahashi, T. et al. (1999) Cell 99:59.
6. Yoshida, Y. et al. (2006) Neuron 52:775.
7. Toyofuku, T. et al. (2005) Nat. Neurosci. 8:1712.
8. Nawabi, H. et al. (2010) Genes Dev. 24:396.
9. Eun, S-Y. et al. (2006) J. Immunol. 177:4271.
10. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
11. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
12. Catalano, A. et al. (2009) Cancer Res. 69:1485.
13. Delloye-Bourgeois, C. et al. (2015) Nat Neurosci 18:36.

Bioinformatics

• Uniprot:
  • Q9UIW2.3()