Recombinant Human NPRC/NPR3 Fc Chimera Protein, CF

When Recombinant Human NPRC/NPR3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 μg/mL, Recombinant Human Osteocrin (Catalog # 9669-ON) binds with an ED50 of 2‑20 ng/mL.

2 μg/lane of Recombinant Human NPRC/NPR3 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-92 kDa and 160-200 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human NPRC/NPR3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human NPRC/NPR3 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Osteocrin (Catalog # 9669-ON) that produces 50% of the optimal binding response is 2-20 ng/mL
• Source: Human embryonic kidney cell, HEK293-derived human NPRC/NPR3 protein
  

  Human NPRC/NPR3 (Thr24-Glu481) Accession # P17342.2	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P17342.2
• N-terminal Sequence: Thr24 & Glu46
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 77 kDa & 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 78-92 kDa

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NPRC/NPR3 Fc Chimera Protein, CF

• ANP-C
• ANPR-C
• ANPRCFLJ14054
• Atrial natriuretic peptide clearance receptor
• atrial natriuretic peptide receptor 3
• Atrial natriuretic peptide receptor type C
• C5orf23
• chromosome 5 open reading frame 23
• GUCY2B
• lgj
• natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C)
• NPR3
• NPRC
• NPR-C
• stri

Background

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPRC or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mature human NPR3 is a type I transmembrane glycoprotein that contains a 455 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 37 aa cytoplasmic region. Within the ECD, human NPR3 shares 92% aa sequence identity with mouse and rat NPR3. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).

1. Potter, L.R. et al. (2006) Endocr. Rev. 27:47.
2. Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081.
3. Thomas, G. et al. (2003) J. Biol. Chem. 278:50563.
4. Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.

Bioinformatics

• Uniprot:
  • P17342.2()