Recombinant Human MIS RII Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human MIS RII Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind rhMIS (Catalog # 1737-MS) in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived human MIS RII protein
  

  Human MIS RII (Pro18 - Ser144) Accession # Q16671	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q16671
• N-terminal Sequence: Pro18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: AMHR2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 40.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MIS RII Fc Chimera Protein, CF

• AMH type II receptor
• AMHR2
• AMHREC 2.7.11.30
• AMHRII
• C14
• MIS RII
• MISR2
• MISRII
• MISRIIMIS type II receptor
• MRII
• Muellerian hormone type II receptor
• Muellerian hormone type-2 receptor
• Mullerian hormone receptor, type II
• Mullerian inhibiting substance type II receptor

Background

Human MIS RII (Mullerian inhibiting substance type II receptor), also known as AMHRII (anti-Mullerian hormone type II receptor), is an 82 kDa serine/threonine receptor with a single transmembrane domain that belongs to the family of type II receptors of the TGF-beta superfamily (1). The MIS RII precursor is 573 amino acids (aa) in length, with a 17 aa signal sequence, a 127 aa extracellular region that also contains two potential N-linked glycosylation sites, a 26 aa transmembrane region, and a 403 aa cytoplasmic region that contains the serine/threonine kinase domain (1). Human MIS RII shares 82%, 78%, and 77% aa sequence identity with rabbit, mouse, and rat MIS RII, respectively. It is expressed in the mesenchyme surrounding the fetal Mullerian duct, in fetal and postnatal granulosa cells, and in Sertoli cells (1 - 6). MIS RII is a receptor for Mullerian inhibitor substance (MIS), also known as anti-Mullerian hormone (AMH), which is responsible for regression of the Mullerian duct, the anlagen of the uterus, Fallopian tubes, and upper vagina in male fetuses (1 - 6). Mutations in MIS RII result in persistent Mullerian duct syndrome (PMDS), an extremely rare form of pseudohermaphroditism (5, 6).

1. Imbeaud, S. et al. (1995) Nat. Genet. 11:382.
2. Baarends, W.M. et al. (1994) Development 120:189.
3. di Clemente, N. et al. (1994) Mol. Endocrinol. 8:1006.
4. Teixeira, J. et al. (1996) Endocrinology 137:160.
5. Salhi, I et al. (2004) Biochem. J. 379:785.
6. Imbeaud, S. et al. (1996) Hum. Mol. Genet. 5:1269.

Publications for MIS RII/AMHR2 (4749-MR)(1)

Publication using 4749-MR

• Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011-07-07;286(34):30034-46. 2011-07-07 [PMID: 21737454] (Surface Plasmon Resonance, Human)

Review for MIS RII/AMHR2 (4749-MR) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer Binding assay/Protein-protein interaction 09/30/2018

Summary

• Application: Binding assay/Protein-protein interaction
• Lot: RND0315091

Bioinformatics

• Gene Symbol: AMHR2
• Uniprot:
  • Q16671()