Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind rhNKG2D/Fc Chimera in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived human MICB protein
  

  Human MICB (Ala23-Gly298) (Asn74Asp) & (Glu24-Gly298) (Asn74Asp) Accession # NP_005922	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_005922
• N-terminal Sequence: Ala23 & Glu24
• Protein/Peptide Type: Recombinant Proteins
• Gene: MICB
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 82 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF

• MHC class I chain-related protein B
• MHC class I mic-B antigen
• MHC class I polypeptide-related sequence B
• MICB
• MIC-B
• PERB11.2MHC class I-like molecule PERB11.2-IMX
• stress inducible class I homolog

Background

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Salih, H. et al. (2003) Blood 102:1389.

Publications for MICB (1599-MB)(4)

Publications using 1599-MB

• S Weil, S Memmer, A Lechner, V Huppert, A Giannattas, T Becker, A Müller-Run, K Lampe, D Beutner, A Quaas, R Schubert, E Herrmann, A Steinle, U Koehl, L Walter, MS von Bergwe, J Koch Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma Front Immunol, 2017;8(0):387. 2017 [PMID: 28443091] (Bioassay)
• AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017;8(19):31959-31976. 2017 [PMID: 28404876] (ELISA (Standard), Human)
• Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J. Mol. Biol., 2008;384(5):1143-56. 2008 [PMID: 18809410] (Bioassay, Human)
• Spreu J, Stehle T, Steinle A Human cytomegalovirus-encoded UL16 discriminates MIC molecules by their alpha2 domains. J. Immunol., 2006;177(5):3143-9. 2006 [PMID: 16920952] (Binding Assay, Human)

Bioinformatics

• Gene Symbol: MICB
• Uniprot:
  • NP_005922()