Recombinant Human Meteorin-like/METRNL Protein, CF

Recombinant Human Meteorin-like/METRNL Induces Stat3 Phosphorylation in E16-E18 RatCortical Neurons. Treatments of E16-E18 rat cortical neuronswith 1 μg/ml (1) and 5 μg/ml of Recombinant Human Meteorin-like/METRNL (2) (Catalog #9339-MN) for 15 mintues results in Stat3 phosphorlation. Untreated E16-E18 rat cortical neurons (3).

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Meteorin-like/METRNL Protein, CF Summary

• Details of Functionality: Measured by its ability to induce STAT3 phosphorylation in E16-E18 embryonic rat cortical neurons. 5 μg/mL of Recombinant Human Meteorin-like/METRNL protein significantly induces phosphorylation of STAT3.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Meteorin-like/METRNL protein
  

  Human Meteorin-like/METRNL (Gln46-Asp311) Accession # Q641Q3	HP	GGGSGGGSGGGS	HHHHHH
  N-terminus			C-terminus

• Accession #: Q641Q3
• N-terminal Sequence: No results obtained. Gln46 inferred from enzymatic pyroglutamate treatment revealing Tyr47
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 14 days, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Meteorin-like/METRNL Protein, CF

• Meteorin like
• meteorin, glial cell differentiation regulator-like
• meteorin-like protein
• Meteorin-like
• METRNL
• MGC99788

Background

Meteorin-like protein (METRNL, Subfatin, Cometin and Interleukin 39) is a 311 amino acid (aa) protein that shows 58% similarity with Meteorin (in human). Human Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide with the secreted mature protein ~30 kDa (1). Mature human Meteorin‑like protein shares 79% and 80% aa sequence identity with mouse and rat METRNL, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide (1). Metrnl is expressed in various adult mouse tissues with the highest expression in muscle tissue, adipose tissue, and activated monocytes (2, 3). Its expression is induced in muscle after exercise and in adipose tissue upon cold exposure suggesting that this secreted protein is both a myokine and an adipokine (4). Neurotrophic activity has been ascribed to Metrnl as it enhances neurite outgrowth and neuroblast migration in vivo. This effect is mediated by the Jak-STAT3 and MEK-ERK pathways (5). Metrnl has also been shown to be indirectly involved in the "browning" of white fat to a more thermogenic "beige fat" through activation of the STAT6 pathway in adipose tissue macrophages, inducing the M2-regulatory cytokines IL-4 and IL-13 (6). Metrnl transgenic mice have demonstrated a role for Metrnl in insulin sensitization through up-regulation of PPAR gamma , a key transcription factor regulating adipocyte differentiation. PPAR gamma increases adipocyte differentiation, inhibits adipose inflammation, activates lipid metabolism, and sensitizes adipocytes to insulin (4).

1. Accession # Q641Q3.
2. Li, ZY et al. (2014) CNS Neurosci Ther. 20:344.
3. Ushach, I et al. (2015) Clin Immunol. 156:119.
4. Zheng, S. et al. (2016) Acta Pharmacol Sin. 37:571.
5. Jorgensen, J.R. et al. (2012) Exp Neurol. 233:172.
6. Rao, RR. et al. (2014) Cell. 157:1279.

Publications for Meteorin-like/METRNL (9339-MN)(2)

Publications using 9339-MN

• Li, J;Ge, Z;Li, C;Ran, H;Zhang, Y;Xiang, Y; METRNL exerts cytoprotective effects on EPCs via regulation of the E2F1-TXNIP axis in obese limb ischemia Cellular signalling 2024-11-26 [PMID: 39603439] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • Q641Q3()