Recombinant Human MDM2/HDM2 Protein, CF

Product Discontinued

Recombinant Human MDM2/HDM2 Protein, CF Summary

• Details of Functionality:

  Recombinant Human Mdm2/Hdm2 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Mdm2/Hdm2 concentration of 0.5-5 μM.

• Source: E. coli-derived human MDM2/HDM2 protein
  Met1 - Pro491
• Accession #: Q00987.1
• Protein/Peptide Type: Recombinant Enzymes
• Gene: MDM2
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MDM2/HDM2 Protein, CF

• ACTFS
• HDM2
• HDMX
• MDM2 oncogene, E3 ubiquitin protein ligase
• MDM2

Background

Double minute 2 protein (Mdm2, also known as Hdm2) is a RING-finger Ubiquitin E3 ligase that acts as a major regulator of the tumor suppressor p53. Mdm2 inhibits p53-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. The E3 ligase activity is confined to the C-terminal domain of Mdm2 and is responsible for the ubiquitinylation and subsequent proteasomal degradation of p53. Although the isolated RING domain is capable of p53 ubiquitinylation, other regions of the protein including a central acidic domain are also crucial for full E3 ligase activity. Mdm2 also regulates its own intracellular levels by auto-ubiquitinylation, and can be SUMOylated which decreases autoubiquitinylation activity but increases activity toward p53. Mdm2 also affects the cell cycle and apoptosis through interactions with other proteins such as retinoblastoma1 (pRB) and ribosomal protein L5. 

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Publications for MDM2/HDM2 (E3-204)(5)

Publications using E3-204

• Sola, M;Rendon-Angel, A;Rojo Martinez, V;Sgrignani, J;Magrin, C;Piovesana, E;Cavalli, A;Paganetti, P;Papin, S; Tau protein binds to the P53 E3 ubiquitin ligase MDM2 Scientific reports 2023-06-23 [PMID: 37353565] (Surface Plasmon Resonance, Human)
• J Li, Y Wang, Y Luo, Y Liu, Y Yi, J Li, Y Pan, W Li, W You, Q Hu, Z Zhao, Y Zhang, Y Cao, L Zhang, J Yuan, ZJ Xiao USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicity Nature Communications, 2022;13(1):7799. 2022 [PMID: 36528652] (Bioassay, Human)
• J Li, Y Wang, Y Luo, Y Liu, Y Yi, J Li, Y Pan, W Li, W You, Q Hu, Z Zhao, Y Zhang, Y Cao, L Zhang, J Yuan, ZJ Xiao USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicity Nature Communications, 2022-12-17;13(1):7799. 2022-12-17 [PMID: 36528652] (Bioassay, Human)
• EM Brydon, R Bronstein, A Buskin, M Lako, EA Pierce, R Fernandez- AAV-Mediated Gene Augmentation Therapy Restores Critical Functions in Mutant PRPF31+/- iPSC-Derived RPE Cells Mol Ther Methods Clin Dev, 2019-11-11;15(0):392-402. 2019-11-11 [PMID: 31890732] (Cell Culture, Human)
• Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity Nucleic Acids Res., 2017-02-28;0(0):. 2017-02-28 [PMID: 27903893] (Bioassay)

Bioinformatics

• Gene Symbol: MDM2
• Uniprot:
  • Q00987.1()