Recombinant Human LRFN5 Fc Chimera Protein, CF Summary
Details of Functionality |
Measured by its binding ability in a functional ELISA. When Recombinant Human LRFN5 Fc Chimera
is used at 1 μg/mL, the concentration of Recombinant
Human LAR that produces 50% optimal binding response is 1.5-9 μg/mL. |
Source |
Human embryonic kidney cell, HEK293-derived human LRFN5 protein Human LRFN5 (Gln18-Gly527) Accession # Q96NI6 | IEGRMD | Human IgG1 (Pro100-Lys330) | N-terminus | | C-terminus | |
|
Accession # |
|
N-terminal Sequence |
No results obtained. Gln18
inferred from enzymatic pyroglutamate treatment revealing Ile19 |
Structure / Form |
Disulfide-linked homodimer |
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
83 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
90-107 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human LRFN5 Fc Chimera Protein, CF
Background
Synaptic
adhesion-like molecule 5 (SALM5; also leucine-rich repeat and fibronectin
type-III domain-containing protein 5 (LRFN5)) is an approximately 90 kDa
member of the SALM family of type I transmembrane glycoproteins (1).
LRFNs comprise a family of synaptic adhesion molecules consisting of five
members, each containing of an extracellular domain (ECD) of six leucine-rich
repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly
aligned in that order (1, 2). LRFN-3 and -5 lack a C-terminal
intracellular PDZ binding domain, which is conserved among LRFN-1, 2 and 4. Mature human LRFN-5 shares 99% amino acid sequence identity with mature mouse
LRFNs. LRFN-5, like the other LRFNs, promotes neurite outgrowth as well as
playing a role in neuroinflammation (3, 4). LRFN-5 is expressed in the
brain and is capable of inducing presynaptic differentiation (5). Reduced
expression of LRFN-5 has been associated with autism spectrum disorders and
schizophrenia (6, 7). LAR family receptor protein tyrosine phosphatases
(LAR-RPTPs) have been identified as novel ligands of LRFN-5 that mediates LRFN-5
dependent presynaptic differentiation in a splicing- dependent manner. LRFN-5 interacts directly with the Ig domain of
LAR-RPTPs. The postsynaptic LRFN-5 promotes synapse
development by trans-synaptically interacting with presynaptic LAR-RPTPs which is important for the regulation of excitatory synaptic strength (8).
-
Morimura, N. et al. (2006) Gene 380:72.
- Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
- Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.
- Yuwen, Z. et al. (2106) Sci Adv. Apr;2(4).
- Choi, Y. et al. (2016) Sci Adv. Apr; 2(4).
- de Bruijn D.R.H. et al. (2010) Mol Syndromol 46.
- Xu, B. et al. (2009) PNAS 106:16746.
- Choi, Y. et.al. (2016) Sci Rep. 6:26676.
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