Recombinant Human KIR3DS1/CD158e2 Fc Chimera Protein, CF

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Recombinant Human KIR3DS1/CD158e2 Fc Chimera (Catalog # 4136-KR)binds toMDA-MB-231 human breast cancer cells. The ED50 for this effect is0.03-0.18 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human KIR3DS1/CD158e2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells. The ED50 for this effect is 0.03-0.18 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human KIR3DS1/CD158e2 protein
Human KIR3DS1
(His22-His340)
Accession #Q14943
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
His22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
KIR3DS1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
61.9 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-90 kDa, reducing conditions
Publications
Read Publications using
4136-KR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KIR3DS1/CD158e2 Fc Chimera Protein, CF

  • CD158e2
  • KIR3DS1
  • KIR-G1
  • nkat10

Background

KIR3DS1 (3DS1, CD158e2) is a type I transmembrane protein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they differentiate normal from abnormal cells, and regulate effector functions in the innate immune system (1 - 3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other activating KIRs, KIR3DS1 has a short cytoplasmic tail and a positively charged amino acid (aa) within the transmembrane domain that interacts with the ITAM-bearing signaling adaptor, DAP12 (2, 4). Crosslinking of KIR3DS1 activates cytolysis and induces IFN-gamma production, confirming it to be an activating receptor (4). Approximately 38% of the population expresses KIR3DS1 on the surface of NK cells (1, 4, 5). Variants lacking the N-terminal Ig-like domain and/or with substitutions in the cytoplasmic tail have been described (1, 6). The 50 kDa, 387 aa KIR3DS1 shows 97% aa identity with KIR3DL1 within the ECD, and the two segregate as alleles (3, 7). Some activating KIRs bind weakly to the ligands recognized by their corresponding inhibitory KIR. KIR3DS1 does not bind appreciably to cells transfected with ligands for HLA-Bw4 KIR3DL1 (4, 5). However, HIV-infected people who express the combined phenotype of KIR3DS1 with Bw4 alleles that contain an isoleucine at aa 80, show delayed progression to AIDS and fewer AIDS-related opportunistic infections (7, 8). KIR receptors have no structural orthologs in nonprimates, although mouse Ly-49 proteins perform similar functions (2).

  1. Dohring, C. et al. (1996) Immunogenetics 44:227.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Uhrberg, M. et al. (1997) Immunity 7:753.
  4. Carr, W. H. et al. (2007) J. Immunol. 178:647.
  5. O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
  6. Valiante, N. M. et al. (1997) Immunity 7:739.
  7. Martin, M. P. et al. (2002) Nat. Genet. 31:429.
  8. Qi, Y. et al. (2006) PloS Pathog. 2:e79.

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Publications for KIR3DS1/CD158e2 (4136-KR)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Flow Cytometry.


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Bioassay
(2)
Flow Cytometry
(2)
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Bioinformatics

Gene Symbol KIR3DS1
Uniprot