Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein, CF


1 µg/lane of Recombinant Human IL-36 gamma /IL-1F9 (aa 18-169) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-36 gamma/IL-1F9 (Catalog #6835-IL/CF) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 1.5-9 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein, CF Summary

Details of Functionality
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for for this effect is 1.5-9 ng/mL.
E. coli-derived human IL-36 gamma/IL-1F9 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17 kDa, reducing coditions
Read Publications using
6835-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, TCEP and EDTA.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in Water.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein, CF

  • IL-1 epsilon
  • IL-1 H1
  • IL-1 Related Protein 2
  • IL1E
  • IL-1-epsilon
  • IL1F9
  • IL-1F9
  • IL1H1
  • IL-1H1
  • IL-1-Related Protein 2
  • IL1RP2
  • IL-1rp2
  • IL36 gamma
  • IL-36 gamma
  • IL36G
  • interleukin 1 family, member 9
  • interleukin 1-related protein 2
  • Interleukin 36, Gamma
  • Interleukin-1 epsilon
  • interleukin-1 family member 9
  • Interleukin-1 homolog 1
  • Interleukin-36 Gamma


IL-36 gamma [previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family which includes IL‑1 beta , IL‑1 alpha , IL‑1ra, IL‑18, IL‑36 Ra (IL‑1F5), IL-36 alpha (IL‑1F6), IL‑36 beta (IL‑1F8), IL‑37 (IL‑1F7) and IL-1F10 (1‑5). All family members show a 12 beta ‑strand, beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene (2, 3). IL‑36 gamma is an 18‑22 kDa, 169 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation sites (1, 2, 4, 6, 7). Human IL‑36 gamma (aa 18‑169) shares 58%, 59%, 68% and 69% aa sequence identity with mouse, rat, bovine and equine IL‑36 gamma , respectively, and 23‑57% aa sequence identity with other family members. A 134 aa isoform missing aa 19‑53 has been reported (8). Highest levels of IL‑36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to first‑line defense against pathogens in the skin, lungs and digestive tract (2, 3, 6, 9). Its expression is induced by LPS treatment of monocytes, and by IL‑ alpha / beta , IL‑17 or TNF‑ alpha treatment of keratinocytes and bronchial epithelia (1, 6, 7, 9‑11). Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis (1, 6, 11). It is elevated in inflammatory disorders of the lung (such as asthma) and viral infections. Lung IL‑36 gamma and other IL‑36 proteins contribute to neutrophil influx (4, 7, 10). The receptor for IL-36 gamma is a combination of IL‑1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (4, 7, 9). IL‑36 alpha ,  beta  and  gamma all activate NF‑ kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and IL‑36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL‑8 (7, 9, 10).
  1. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  2. Busfield, S.J. et al. (2000) Genomics 66:213.
  3. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  4. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  5. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  6. Debets, R. et al. (2001) J. Immunol. 167:1440.
  7. Chustz, R.T. et al. (2010) Am. J. Respir. Cell Mol. Biol. Sept. 24 [Epub ahead of print]
  8. SwissProt Accession # Q9NZH8.
  9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  10. Ramadas, R.A. et al. (2011) Am. J. Respir. Cell Mol. Biol. 44:134.
  11. Johnston, A. et al. (2011) J. Immunol. 186:2613.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: Block, Neut, WB
Species: Hu
Applications: WB
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, Neut
Species: Hu
Applications: CyTOF-ready, Flow, WB
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Mu
Applications: ELISA
Species: Mu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB

Publications for IL-36 gamma/IL-1F9 (6835-IL/CF)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Cell Culture, Whole Cells.

Filter By Application
Cell Culture
Whole Cells
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 10.
Publications using 6835-IL/CF Applications Species
A Müller, A Dickmanns, C Resch, K Schäkel, S Hailfinger, M Dobbelstei, K Schulze-Os, D Kramer The CDK4/6-EZH2 pathway is a potential therapeutic target for psoriasis J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32701505] (Cell Culture, Human) Cell Culture Human
JE Heath, GM Scholz, PD Veith, EC Reynolds IL-36? regulates mediators of tissue homeostasis in epithelial cells Cytokine, 2019;119(0):24-31. 2019 [PMID: 30856602] (Bioassay, Human) Bioassay Human
J Guo, J Tu, Y Hu, G Song, Z Yin Cathepsin G cleaves and activates IL-36? and promotes the inflammation of psoriasis Drug Des Devel Ther, 2019;13(0):581-588. 2019 [PMID: 30804664] (Bioassay, Human) Bioassay Human
L Mercurio, M Morelli, C Scarponi, EZ Eisenmesse, N Doti, G Pagnanelli, E Gubinelli, C Mazzanti, A Cavani, M Ruvo, CA Dinarello, C Albanesi, S Madonna IL-38 has an anti-inflammatory action in psoriasis and its expression correlates with disease severity and therapeutic response to anti-IL-17A treatment Cell Death Dis, 2018;9(11):1104. 2018 [PMID: 30377293] (Bioassay, Human) Bioassay Human
BA Stanfield, PJF Rider, J Caskey, F Del Piero, KG Kousoulas Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vaginal Th17 and regulatory Tr1 responses Vaccine, 2018;0(0):. 2018 [PMID: 29655629] (Bioassay, Human) Bioassay Human
WR Swindell, MA Beamer, MK Sarkar, S Loftus, J Fullmer, X Xing, NL Ward, LC Tsoi, MJ Kahlenberg, Y Liang, JE Gudjonsson RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature Front Immunol, 2018;9(0):80. 2018 [PMID: 29434599] (Whole Cells, Human) Whole Cells Human
W Wang, X Yu, C Wu, H Jin IL-36? inhibits differentiation and induces inflammation of keratinocyte via Wnt signaling pathway in psoriasis Int J Med Sci, 2017;14(10):1002-1007. 2017 [PMID: 28924372] (Bioassay, Human) Bioassay Human
Guanghui Yi Structural and functional attributes of the Interleukin-36 receptor J Biol Chem, 2016;0(0):. 2016 [PMID: 27307043] (Bioassay, Human) Bioassay Human
Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor. J Biol Chem, 2015;290(39):23997-4006. 2015 [PMID: 26269592] (Bioassay, Human) Bioassay Human
Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury. Sci Rep, 2015;5(0):8521. 2015 [PMID: 25687687] (Bioassay, Human) Bioassay Human

Reviews for IL-36 gamma/IL-1F9 (6835-IL/CF) (0)

There are no reviews for IL-36 gamma/IL-1F9 (6835-IL/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-36 gamma/IL-1F9 (6835-IL/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-36 gamma/IL-1F9 Products

Bioinformatics Tool for IL-36 gamma/IL-1F9 (6835-IL/CF)

Discover related pathways, diseases and genes to IL-36 gamma/IL-1F9 (6835-IL/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-36 gamma/IL-1F9 (6835-IL/CF)

Discover more about diseases related to IL-36 gamma/IL-1F9 (6835-IL/CF).

Pathways for IL-36 gamma/IL-1F9 (6835-IL/CF)

View related products by pathway.

Blogs on IL-36 gamma/IL-1F9

There are no specific blogs for IL-36 gamma/IL-1F9, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein, CF and receive a gift card or discount.


Gene Symbol IL36G