Recombinant Human IL-29/IFN-lambda 1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-29/IFN-lambda 1 Protein Summary

Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 1-5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-29/IFN-lambda 1 protein
Gly20-Thr200, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Gly20
Protein/Peptide Type
Recombinant Proteins
Gene
IFNL1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
26-35 kDa, reducing conditions
Publications
Read Publications using
1598-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-29/IFN-lambda 1 Protein

  • cytokine Zcyto21
  • IFNL1
  • IFN-lambda 1
  • IFN-lambda-1
  • IL29
  • IL-29
  • interferon lambda-1
  • interferon, lambda 1
  • interleukin 29 (interferon, lambda 1)
  • interleukin-29
  • ZCYTO21

Background

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor  beta (IL-10 R beta ) and a novel IL-28 receptor  alpha (IL‑28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.
  1. Vilcek, J. (2003) Nature Immunol. 4:8.
  2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

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Publications for IL-29/IFN-lambda 1 (1598-IL)(28)

We have publications tested in 2 confirmed species: Human, Primate - Chlorocebus aethiops (African Green Monkey).

We have publications tested in 5 applications: Bioassay, Cell Culture, Control, ELISA (Standard), Stimulation.


Filter By Application
Bioassay
(22)
Cell Culture
(1)
Control
(1)
ELISA (Standard)
(2)
Stimulation
(1)
All Applications
Filter By Species
Human
(26)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 1598-IL Applications Species
JH Chung, SH Hong, N Seo, TS Kim, HJ An, P Lee, EC Shin, HM Kim Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency Cytokine, 2019;125(0):154833. 2019 [PMID: 31479875] (Bioassay, Human) Bioassay Human
C Good, AI Wells, CB Coyne Type III interferon signaling restricts enterovirus 71 infection of goblet cells Sci Adv, 2019;5(3):eaau4255. 2019 [PMID: 30854425] (Bioassay, Human) Bioassay Human
H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway Elife, 2019;8(0):. 2019 [PMID: 30735121] (Bioassay, Human) Bioassay Human
I Angelidou, A Chrysantho, A Mitsios, S Arelaki, A Arampatzio, K Kambas, D Ritis, V Tsironidou, I Moschos, V Dalla, D Stakos, G Kouklakis, I Mitroulis, K Ritis, P Skendros REDD1/Autophagy Pathway Is Associated with Neutrophil-Driven IL-1? Inflammatory Response in Active Ulcerative Colitis J. Immunol., 2018;0(0):. 2018 [PMID: 29712770] (Bioassay, Human) Bioassay Human
A Pierangeli, M Statzu, R Nenna, L Santinelli, L Petrarca, A Frassanito, M Gentile, G Antonelli, F Midulla, C Scagnolari Interferon lambda receptor 1 (IFNL1R) transcript is highly expressed in rhinovirus bronchiolitis and correlates with disease severity J. Clin. Virol., 2018;102(0):101-109. 2018 [PMID: 29549834] (Stimulation, Human) Stimulation Human
SS Kwek, S Watanabe, KR Chan, EZ Ong, HC Tan, WC Ng, MTX Nguyen, ES Gan, SL Zhang, KWK Chan, JH Tan, OM Sessions, M Manuel, J Pompon, C Chua, S Hazirah, K Tryggvason, SG Vasudevan, EE Ooi A systematic approach to the development of a safe live attenuated Zika vaccine Nat Commun, 2018;9(1):1031. 2018 [PMID: 29531213] (Cell Culture, Primate - Chlorocebus aethiops (African Green Monkey)) Cell Culture Primate - Chlorocebus aethiops (African Green Monkey)
AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling J. Immunol., 2017;0(0):. 2017 [PMID: 29070670] (Bioassay, Human) Bioassay Human
J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28784796] (Bioassay, Human) Bioassay Human
NA Ilyushina, VY Lugovtsev, AP Samsonova, FG Sheikh, NV Bovin, RP Donnelly Generation and characterization of interferon-lambda 1-resistant H1N1 influenza A viruses PLoS ONE, 2017;12(7):e0181999. 2017 [PMID: 28750037] (Bioassay, Human) Bioassay Human
PS Sung, SH Hong, JH Chung, S Kim, SH Park, HM Kim, SK Yoon, EC Shin IFN-?4 potently blocks IFN-? signalling by ISG15 and USP18 in hepatitis C virus infection Sci Rep, 2017;7(1):3821. 2017 [PMID: 28630501] (Bioassay, Human) Bioassay Human
Show All 28 Publications.

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Bioinformatics

Gene Symbol IFNL1
Uniprot