Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein Summary
Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 5-25 ng/mL.
Human embryonic kidney cell, HEK293-derived human IL-28A/IFN-lambda 2 protein Val26-Val200
<0.10 EU per 1 μg of the protein by the LAL method.
20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE with silver staining
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein
interferon, lambda 2
IL‑28A (Interferon-lambda 2; IFN-lambda 2), IL-28B/IFN-lambda 3, and IL-29/IFN-lambda 1 are type III interferons which are distantly related to IL-10 family and type I IFN family cytokines (1‑3). Mature human IL-28A is an approximately 22-25 kDa protein that shares 66% amino acid (aa) sequence identity with mouse and rat IL-28A and shows cross-species activity (4). It shares 96% and 70% aa sequence identity with human IL-28B and IL-29, respectively. Type III interferons are upregulated in response to viral infection, and they act predominantly on epithelial cells through a receptor complex that contains IL-10 R beta and IL-28 R alpha /IFN-gamma R1 (4-6). Type III IFNs function as anti-viral molecules and induce the upregulation of MHC class I antigen (4, 5, 7). IL-28A promotes the Th1 polarization of dendritic cells in the airway (e.g. production of IL-12 p70 and IFN-gamma ) and inhibits Th2 and Th17 mediated inflammation (8). In the liver, however, the IL-28A induced Th1 cytokine response contributes to inflammation in T cell mediated hepatitis (9). IL-28A additionally exhibits anti-tumor activity, in part by enhancing IL-12 dependent anti-tumor CTL responses in vivo (10-12). In contrast, it is up-regulated in invasive bladder cancer where it promotes tumor cell migration (13).
Durbin, R.K. et al. (2013) Immunol. Rev. 255:25.
Li, Q. et al. (2013) Front. Biosci. (Landmark Ed.) 18:909.
Lopusna, K. et al. (2013) Acta Virol. 57:171.
Ank, N. et al. (2006) J. Virol. 80:4501.
Sheppard, P. et al. (2003) Nature Immunol. 4:63.
Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
Pott, J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:7944.
Koltsida, O. et al. (2011) EMBO Mol. Med. 3:348.
Siebler, J. et al. (2007) Gastroenterology 132:358.
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