Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein

Images

 
There are currently no images for IL-28A/IFN-lambda 2 (8417-IL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein Summary

Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 5-25 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-28A/IFN-lambda 2 protein
Val26-Val200
Accession #
N-terminal Sequence
Val26 & Val28
Protein/Peptide Type
Recombinant Proteins
Gene
IFNL2
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-23 kDa, reducing condtions
Publications
Read Publications using
8417-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein

  • interleukin-28A
  • IFN-lambda 2
  • IL28A
  • IL-28A
  • interferon, lambda 2

Background

IL‑28A (Interferon-lambda 2; IFN-lambda 2), IL-28B/IFN-lambda 3, and IL-29/IFN-lambda 1 are type III interferons which are distantly related to IL-10 family and type I IFN family cytokines (1‑3). Mature human IL-28A is an approximately 22-25 kDa protein that shares 66% amino acid (aa) sequence identity with mouse and rat IL-28A and shows cross-species activity (4). It shares 96% and 70% aa sequence identity with human IL-28B and IL-29, respectively. Type III interferons are upregulated in response to viral infection, and they act predominantly on epithelial cells through a receptor complex that contains IL-10 R beta and IL-28 R alpha /IFN-gamma R1 (4-6). Type III IFNs function as anti-viral molecules and induce the upregulation of MHC class I antigen (4, 5, 7). IL-28A promotes the Th1 polarization of dendritic cells in the airway (e.g. production of IL-12 p70 and IFN-gamma ) and inhibits Th2 and Th17 mediated inflammation (8). In the liver, however, the IL-28A induced Th1 cytokine response contributes to inflammation in T cell mediated hepatitis (9). IL-28A additionally exhibits anti-tumor activity, in part by enhancing IL-12 dependent anti-tumor CTL responses in vivo (10-12). In contrast, it is up-regulated in invasive bladder cancer where it promotes tumor cell migration (13).
  1. Durbin, R.K. et al. (2013) Immunol. Rev. 255:25.
  2. Li, Q. et al. (2013) Front. Biosci. (Landmark Ed.) 18:909.
  3. Lopusna, K. et al. (2013) Acta Virol. 57:171.
  4. Ank, N. et al. (2006) J. Virol. 80:4501.
  5. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  6. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
  7. Pott, J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:7944.
  8. Koltsida, O. et al. (2011) EMBO Mol. Med. 3:348.
  9. Siebler, J. et al. (2007) Gastroenterology 132:358.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Tezuka, Y. et al. (2012) Lung Cancer 78:185.
  12. Numasaki, M. et al. (2007) J. Immunol. 178:5086.
  13. Lee, S.J. et al. (2012) PLoS ONE 7:e40267.

Customers Who Viewed This Item Also Viewed...

NBP2-75930
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF874
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
NBP1-32905
Species: Hu, Bv, Mk, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
MAB15041
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF5260
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
AF1709
Species: Mu
Applications: WB, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ICFlow, ELISA(Sta)
7270-IL/CF
Species: Hu
8417-IL
Species: Hu
Applications: Bioactivity

Publications for IL-28A/IFN-lambda 2 (8417-IL)(2)

Reviews for IL-28A/IFN-lambda 2 (8417-IL) (0)

There are no reviews for IL-28A/IFN-lambda 2 (8417-IL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-28A/IFN-lambda 2 (8417-IL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-28A/IFN-lambda 2 Products

Bioinformatics Tool for IL-28A/IFN-lambda 2 (8417-IL)

Discover related pathways, diseases and genes to IL-28A/IFN-lambda 2 (8417-IL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on IL-28A/IFN-lambda 2

There are no specific blogs for IL-28A/IFN-lambda 2, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol IFNL2
Uniprot