Recombinant Human IL-26/AK155 Dimer Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-26/AK155 Dimer Protein Summary

Details of Functionality
Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. Hor, S. et al. (2004) J. Biol. Chem. 279:33343. The ED50 for this effect is 10-75 ng/mL.
Source
E. coli-derived human IL-26/AK155 protein
Lys22-Gln171, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Structure / Form

Disulfide-linked homodimer

Protein/Peptide Type
Recombinant Proteins
Gene
IL26
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18 kDa, reducing conditions
36 kDa, non-reducing conditions
Publications
Read Publications using
1870-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in NaH2PO4, NaCl and Glycerol with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-26/AK155 Dimer Protein

  • AK155
  • AK155IL-26interleukin-26
  • IL26
  • IL-26
  • interleukin 26
  • Protein AK155

Background

IL-26 was originally cloned from herpesvirus saimiri (HVS)-transformed T-cells and named AK155 (1). It is a member of the IL-10 family of class II cytokines that signal via heterodimeric receptor complexes composed of two type I transmembrane receptor subunits (2). The human IL-26 gene has been mapped to chromosome 12q15. It encodes a 171 amino acid polypeptide with a 21 amino acid signal peptide. In addition to HVS-transformed T cells, IL-26 is also expressed in other virus transformed T cell lines, fresh peripheral mononuclear cells, activated NK cells and T cells. A mouse homologue of human IL-26 has not been identified. IL-26 binds with high-affinity to the heterodimeric complex consisting of the ligand-binding IL-20 R alpha and non ligand-binding IL-10 R beta (3). Activation of the receptor complex results in rapid phosphorylation of STAT1 and STAT3. Although the IL-26 receptor complex is highly specific for IL-26 and is not activated by other class II cytokines, the individual subunits of the IL-26 receptor complex are components in receptor complexes for other class II cytokines (1). IL-20 R alpha can form dimers with IL‑20 R beta to function as signaling receptors for IL-19, IL-20, and IL-24. IL-10 R beta  can complex with IL-10 R alpha, IL-22 R, and IL-28 R alpha  to transduce signals for IL-10, IL-22, and the three novel IFNs (IL-28A, IL-28B and IL-29), respectively. The physiological functions of IL-26 remain to be determined.

IL-26 was reported to be a homodimer in solution (1). E. coli-derived IL-26 produced by R&D Systems contains disulfide-linked homodimers and monomers. Both the pure monomeric and dimeric IL-26 can bind directly to IL-20 R alpha  and induce STAT activation in COLO-205 cells (4). Besides IL-20 R alpha, R&D Systems IL-26 preparations have also been shown to bind IL‑20 R beta. The significance of this receptor-ligand interaction has not been investigated.

  1. Knappe. A. et al. (2000) J. Virology 74:3881.
  2. Renauld, J.-C. (2003) Nature Reviews Immunology 3:667.
  3. Sheikh, F. et al. (2004) J. Immunol. 172:2006.
  4. Unpublished data (2003) R&D Systems, Inc.

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Publications for IL-26/AK155 (1870-IL)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Binding Assay, Bioassay, In Vivo.


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Binding Assay
(1)
Bioassay
(2)
In Vivo
(1)
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Human
(3)
Mouse
(1)
All Species

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Bioinformatics

Gene Symbol IL26
Uniprot