Recombinant Human IL-20 R beta Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IL-20 R beta Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑19 (Catalog # 1035‑IL) is present at 0.1 μg/mL, the concentration of Recombinant Human IL‑20 R beta Fc Chimera that produces 50% of the optimal binding response is approximately 10‑50 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human IL-20 R beta protein
  

  Human IL-20 R beta (Asp30-Ala230) Accession # Q6UXL0	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q6UXL0
• N-terminal Sequence: Asp30
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL20RB
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 49.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-20 R beta Fc Chimera Protein, CF

• FNDC6
• DIRS1
• FNDC6
• IL-20 R beta
• IL20R beta
• IL-20R2
• IL20RB interleukin 20 receptor beta
• IL20RB
• IL-20Rb
• MGC34923

Background

IL-20 receptor beta (IL-20 R beta ), also known as IL-20 R2, is a transmembrane glycoprotein in the class II cytokine receptor family. These receptors are characterized by tandem fibronectin type III domains in their extracellular region and the lack of a WSXWS motif (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). Human IL-20 R beta consists of a 204 aa extracellular domain (ECD) with two fibronectin type III domains, a 21 aa transmembrane segment, and a 57 aa cytoplasmic domain (2). Within the ECD, human IL-20 R beta shares 81% aa sequence identity with mouse IL-20 R beta . It shares 15%-28% aa sequence identity with the human class II cytokine receptors IFN‑ alpha  R1, IFN-alpha R2, IFN-gamma R1, IFN-gamma R2, IL-10 R alpha , IL-10 R beta , IL-20 R alpha , IL-22BP, IL-22 R alpha , IL-28 R, and tissue factor. Strong IL-20 R beta expression is normally restricted to skin and testis (3). IL-20 R beta is also expressed in psoriatic skin, rheumatoid arthritis synovial membranes, and hepatocytes of LPS-treated mice, and it contributes to the local inflammatory reaction (3-6). IL-20 R beta heterodimerizes with IL-20 R alpha to form the receptor complex that mediates IL-19, IL-20 and IL-24 signals (3, 7-10). It also heterodimerizes with IL-22 R to form the functional receptor complex for IL-20 and IL-24 (7-9). Binding of these IL-10 family class II cytokines to their receptors induces activation of the JAK-STAT signal transduction pathway.

1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
2. SwissProt # Q6UXL0.
3. Blumberg, H. et al. (2001) Cell 104:9.
4. Hsu, Y.-H. et al. (2006) Arthritis Rheum. 54:2722.
5. Wegenka, U.M. et al. (2007) J. Hepatol. 46:257.
6. Chan, J.R. et al. (2006) J. Exp. Med. 203:2577.
7. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
8. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
9. Wang, M., et al. (2002) J. Biol. Chem. 277:7341.
10. Pletnev, S. et al. (2003) Biochemistry 42:12617.

Bioinformatics

• Gene Symbol: IL20RB
• Uniprot:
  • Q6UXL0()