Recombinant Human IGFLR1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGFLR1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IGFLR1 is coated at 0.1 μg/mL (100 μL/well), the concentration of recombinant human IGFL1 that produces 50% optimal binding response is 0.6-3 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IGFLR1 protein
  Ser23-Pro163 with a C-terminal 6-His tag
• Accession #: Q9H665
• N-terminal Sequence: Ser23
• Protein/Peptide Type: Recombinant Proteins
• Gene: IGFLR1
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 22-32 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGFLR1 Protein, CF

• FLJ22573
• IGFLR1
• TMEM149
• transmembrane protein 149
• U2 small nuclear RNA auxiliary factor 1-like 4
• U2(RNU2) small nuclear RNA auxiliary factor 1-like 4
• U2AF1L4

Background

Insulin Growth Factor-like Family Receptor 1 (IGFLR1) is a 355 amino acid (aa) type 1a transmembrane protein that was identified in a screen for binding partners of human IGFL1 (1). Mature human IGFLR1 consists of an extracellular domain (ECD) with two putative cysteine-rich domains (CRDs), a transmembrane region, and a cytoplasmic domain (1). Its structure has similarities to TNF receptor family members (1).  The ECD contains 12 cysteine residues that are conserved among humans, macaque, mice, and rats (1). Over the first 163 aa, human IGFLR1 shares 61% and 59% aa sequence identity with mouse and rat IGFLR1, respectively. In mice, IGFLR1 is expressed primarily on T cells and, similar to the human proteins, mouse IGFLR1 binds the mouse IGFL protein (1). Human IGFL1 expression is enhanced by TNF-alpha treatment and was shown to be up-regulated in human psoriatic skin samples, suggesting that IGFLR1 may have a role during skin inflammation (1).

1. Lobito, A.A. et al. (2011) J. Biol. Chem. 286:18969.

Bioinformatics

• Gene Symbol: IGFLR1
• Uniprot:
  • Q9H665()