Recombinant Human IFN-alpha A (alpha 2a) Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA

Order Details

Recombinant Human IFN-alpha A (alpha 2a) Protein Summary

Details of Functionality

Interferon was titrated with the use of the cytopathic effect inhibition assay as described:

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.

The units are determined by use of a Human IFN Alpha A (Hu-IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for human interferon alpha A (Hu-IFN- alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

Source
E. coli-derived
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
IFNA2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
19.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
11100-1 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Buffer

Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-alpha A (alpha 2a) Protein

  • IFNA2a
  • IFNalpha A
  • IFN-alpha A

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

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Publications for (7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Ctrl.


Filter By Application
Bioassay
(6)
Ctrl
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using 11100-1 Applications Species
JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study PLoS ONE, 2017;12(6):e0179981. 2017 [PMID: 28665954] (Ctrl, Human) Ctrl Human
Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production Proc Natl Acad Sci USA, 2016;113(38):10625-10630. 2016 [PMID: 27601648] (Bioassay, Human) Bioassay Human
AM Gomez, M Ouellet, A Deshiere, Y Breton, MJ Tremblay HIV-1-Mediated BAFF Secretion in Macrophages Does Not Require Endosomal TLRs, Type-I IFN, and Nef, but Depends on the Cellular Phenotype Status J Immunol, 2016;0(0):. 2016 [PMID: 27022194] (Bioassay, Human) Bioassay Human
Gomez A, Ouellet M, Tremblay M HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes. J Immunol, 2015;194(5):2300-8. 2015 [PMID: 25637018] (Bioassay, Human) Bioassay Human
Ding S, Khoury-Hanold W, Iwasaki A, Robek M Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth. PLoS Biol, 2014;12(1):e1001758. 2014 [PMID: 24409098] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Gerber SA, Pober JS IFN-alpha induces transcription of hypoxia-inducible factor-1alpha to inhibit proliferation of human endothelial cells. J. Immunol., 2008;181(2):1052-62. 2008 [PMID: 18606657] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol IFNA2
Entrez
Uniprot