Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF


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Reactivity HuSpecies Glossary
Applications Enzyme Activity

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Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF Summary

Details of Functionality
Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
Contains an N-terminal Met-Ser-Tyr-Tyr and 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain


Theoretical MW
121 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
E-304 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

0.61 mg/ml (5 µM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 1 mM TCEP

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF

  • A1S9T and BN75 temperature sensitivity complementing
  • A1S9T
  • A1ST
  • AMCX1
  • GXP1
  • MGC4781
  • POC20 centriolar protein homolog
  • POC20
  • Protein A1S9
  • SMAX2
  • UBA1
  • UBA1, ubiquitin-activating enzyme E1 homolog A
  • UBA1A
  • UBE1
  • UBE1A1S9
  • UBE1X
  • ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
  • Ubiquitin-activating enzyme E1
  • Ubiquitinactivating Enzyme
  • Ubiquitin-activating Enzyme
  • ubiquitin-like modifier activating enzyme 1
  • ubiquitin-like modifier-activating enzyme 1


Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa.  It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating  – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions. This protein has an N-terminal His6 tag.


  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.

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Publications for Ubiquitin-activating Enzyme/UBE1 (E-304)(29)

We have publications tested in 4 confirmed species: Human, Rat, E. coli, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, Ubiquitination, in vitro ubiquitination.

Filter By Application
Binding Assay
in vitro ubiquitination
All Applications
Filter By Species
E. coli
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using E-304 Applications Species
T Schneider, A Martinez-M, M Cubillos-R, R Bartrons, F Ventura, JL Rosa The E3 ubiquitin ligase HERC1 controls the ERK signaling pathway targeting C-RAF for degradation Oncotarget, 2018;9(59):31531-31548. 2018 [PMID: 30140388] (Bioassay) Bioassay
KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES Fischer Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome Elife, 2018;7(0):. 2018 [PMID: 30067223] (Ubiquitination, Human) Ubiquitination Human
Y Chen, M Fokar, M Kang, N Chen, RD Allen, Y Chen Phosphorylation of Arabidopsis SINA2 by CDKG1 affects its ubiquitin ligase activity BMC Plant Biol., 2018;18(1):147. 2018 [PMID: 30012094] (Bioassay, Human) Bioassay Human
R Magliozzi, ZI Carrero, TY Low, L Yuniati, C Valdes-Que, F Kruiswijk, K van Wijk, AJR Heck, CL Jackson, D Guardavacc Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1 Cell Rep, 2018;23(11):3381-3391.e4. 2018 [PMID: 29898406] (Bioassay) Bioassay
SC Schuyler, YO Wu, HY Chen, YS Ding, CJ Lin, YT Chu, TC Chen, L Liao, WW Tsai, A Huang, LI Wang, TW Liao, JH Jhuo, V Cheng Peptide inhibitors of the anaphase promoting-complex that cause sensitivity to microtubule poison PLoS ONE, 2018;13(6):e0198930. 2018 [PMID: 29883473] (Bioassay) Bioassay
M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H Yao PCGF5 is required for neural differentiation of embryonic stem cells Nat Commun, 2018;9(1):1463. 2018 [PMID: 29765032] (Bioassay, Human) Bioassay Human
F Wojcik, GP Dann, LY Beh, GT Debelouchi, R Hofmann, TW Muir Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants Nat Commun, 2018;9(1):1394. 2018 [PMID: 29643390] (Bioassay) Bioassay
WO Tucker, AB Kinghorn, LA Fraser, YW Cheung, JA Tanner Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1 Int J Mol Sci, 2018;19(3):. 2018 [PMID: 29518962] (Bioassay, Human) Bioassay Human
H Tsuchiya, D Burana, F Ohtake, N Arai, A Kaiho, M Komada, K Tanaka, Y Saeki Ub-ProT reveals global length and composition of protein ubiquitylation in cells Nat Commun, 2018;9(1):524. 2018 [PMID: 29410401] (Bioassay) Bioassay
DS Guimaraes, MD Gomes Expression, purification, and characterization of the TRIM49 protein Protein Expression and Purification, 2018;143(0):57-61. 2018 [PMID: 29107100] (Bioassay, Human) Bioassay Human
Show All 29 Publications.

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Gene Symbol UBA1