Recombinant Human His6-Miro1/Rhot1 Protein, CF

Product Discontinued

Recombinant Human His6-Miro1/Rhot1 Protein, CF Summary

• Details of Functionality: Reaction Conditions will be optimized for each specific application. In a 50 μl reaction containing 1 μM activated Parkin E3 ligase (E3-160 or E3-162), 1 μM His6-Miro1 is fully converted to Ubiquitinated forms in 60 minutes.
• Source: E. coli-derived human Miro1/Rhot1 protein
  Contains an N-terminal 6-His tag, Ile186 - Thr591
• Accession #: Q8IXI2
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-Miro1/Rhot1 Protein, CF

• ARHT1
• EC 3.6.5
• EC 3.6.5.-
• FLJ11040
• FLJ12633
• hMiro-1
• Miro1
• MIRO-1mitochondrial Rho GTPase 1
• mitochondrial Rho 1
• rac-GTP binding protein-like protein
• Rac-GTP-binding protein-like protein
• Ras homolog gene family member T1
• ras homolog gene family, member T1
• Rhot1

Background

Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa.  Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules.  A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria.  This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.

1. Birsa, N. et al. (2014) J. Biol. Chem.  289: 14569
2. Kazlauskaite, A. et al. (2014) Open Biol.  4: 130213
3. Klosowiak, J.L. et al. (2016) Sci. Rep.  6: 33019
4. MacAskill, A.F. et al. (2009)  Neuron  61: 541
5. Ordureau, A. et al. (2015)  Proc. Natl. Acad. Sci.  112: 637

Bioinformatics

• Uniprot:
  • Q8IXI2()