Recombinant Human GST-UbcH5a/UBE2D1 Protein, CF

Product Discontinued

Recombinant Human GST-UbcH5a/UBE2D1 Protein, CF Summary

• Details of Functionality: Recombinant Human GST-UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH5a/UBE2D1 concentration of 0.1-1 μM.
• Source: E. coli-derived human UbcH5a/UBE2D1 protein
  Contains an N-terminal GST (glutathione S-transferase) tag
• Accession #: P51668
• Protein/Peptide Type: Recombinant Enzymes
• Gene: UBE2D1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer:

  X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP

• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GST-UbcH5a/UBE2D1 Protein, CF

• E2(17)KB1
• Stimulator of Fe transportUBC4/5 homolog
• UBC4/5
• UBC5A
• UbcH5
• UbcH5a
• UBCH5AEC 6.3.2.19
• UBCH5SFT
• UBE2D1
• Ubiquitin carrier protein D1
• ubiquitin-conjugating enzyme E2 D1
• Ubiquitin-conjugating enzyme E2(17)KB 1
• Ubiquitin-conjugating enzyme E2-17 kDa 1
• ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D1

Background

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).

 

1. Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
2. Gehrke, S. et al. (2003) Blood 101:3288.
3. Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
4. Lorick, K. et al. (2005) Methods Enzymol. 398:54.
5. Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
6. Dynek, J. et al. (2010) EMBO J. 29:4128.
7. Vanni, E. et al. (2012) J. Virol. 86:6323.

Publications for UbcH5a/UBE2D1 (E2-615)(4)

Publications using E2-615

• Yuan, Y;Qian, L;Miao, Y;Cui, Q;Cao, T;Yu, Y;Zhang, T;Zhao, Q;Zhang, R;Ren, T;Zuo, Y;Du, Q;Qiao, C;Wu, Q;Zheng, Z;Li, M;Chinn, YE;Xu, W;Peng, T;Chen, R;Xiong, S;Zheng, H; Targeting Viperin prevents coxsackievirus B3-induced acute heart failure Cell discovery 2025-04-08 [PMID: 40195316] (Bioassay, N/A)
• M Han, J Li, Y Cao, Y Huang, W Li, H Zhu, Q Zhao, JJ Han, Q Wu, J Li, J Feng, J Wong A role for LSH in facilitating DNA methylation by DNMT1 through enhancing UHRF1 chromatin association Nucleic Acids Res, 2020-12-02;0(0):. 2020-12-02 [PMID: 33170271] (Bioassay, Human)
• Lee , T-H, Park , J-M, Leem , S-H, Kang , T-H Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair. Oncogene, 2012-11-26;33(1):19-25. 2012-11-26 [PMID: 23178497] (Bioassay)
• Janzer , Andreas, Stamm , Katrin, Becker , Astrid, Zimmer , Andreas, Buettner , Reinhard, Kirfel , Jutta The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology, and the metabolome of fibroblasts. J Biol Chem, 2012-07-23;287(37):30984-92. 2012-07-23 [PMID: 22825849] (Bioassay)

Bioinformatics

• Gene Symbol: UBE2D1
• Uniprot:
  • P51668(Human)