Recombinant Human GASP-2/WFIKKN Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human GASP-2/WFIKKN Protein Summary

• Details of Functionality: Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. The ED₅₀ for this effect is 0.25-1.25 µg/mL, in the presence of 40 ng/mL of rmGDF-8.
• Source: Mouse myeloma cell line, NS0-derived human GASP-2/WFIKKN protein
  Ala20-Asp548, with a C-terminal 6-His tag
• Accession #: Q96NZ8
• N-terminal Sequence: Ala20
• Protein/Peptide Type: Recombinant Proteins
• Gene: WFIKKN1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-72 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GASP-2/WFIKKN Protein

• C16orf12
• Chromosome 16 Open Reading Frame 12
• GASP2
• GASP-2
• Growth And Differentiation Factor-Associated Serum Protein 2
• hGASP-2
• RJD2
• WAP Four-Disulfide Core Domain 20A
• WAP, Follistatin/Kazal, Immunoglobulin, Kunitz And Netrin Domain Containing 1
• WAP, FS, Ig, KU, And NTR-Containing Protein
• WFDC20A
• WFIKKN
• WFIKKN1

Background

Growth and differentiation factor-associated serum protein-2 (GASP-2) cDNA encodes a 548 amino acid protein that contains a 19 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Based on the order of these conserved domains, GASP-2 is also known as WFIKKN (1). Another related protein which contains the same domain structure is called WFIKKNRP (WFIKKN-related protein), or GASP-1 (2, 3). WAP, follistatin, Kunitz and netrin domains are all implicated in protease inhibition, and the GASP proteins may be multivalent protease inhibitors (1, 4). Tests at R&D systems have measured the ability of GASP-2 to inhibit trypsin cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (R&D Systems Catalog # ES002). The IC₅₀ value was approximately 10 nM, as measured in a reaction mixture containing 1.0 nM trypsin, 10 µM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-2 is abundant in the lung, skeletal muscle and liver while GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney (3). In the adult, GASP-2 is expressed primarily in the pancreas, liver, and thymus while GASP-1 is in the ovary, testis, and brain (3). Further characterization shows that GASP-1 inhibits myostatin (GDF-8) and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (2). Although, this kind of activity has not been reported for GASP-2, tests at R&D Systems have determined that GASP-2 shows similar inhibitory activity towards myostatin as GASP-1. By amino acid sequence, human GASP-2 is 55% identical to human GASP-1.

1. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3705.
2. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
3. Trexler, M. et al. (2002) Biol. Chem. 383:223.
4. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.

Publications for GASP-2/WFIKKN (2136-GS)(1)

Publication using 2136-GS

• RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson Structural basis for potency differences between GDF8 and GDF11 BMC Biol, 2017-03-03;15(1):19. 2017-03-03 [PMID: 28257634] (Bioassay, Human)

Bioinformatics

• Gene Symbol: WFIKKN1
• Uniprot:
  • Q96NZ8()