Recombinant Human DLL3 Protein, CF

2 μg/lane of Recombinant Human DLL3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 52-62 kDa and 40-55 kDa, respectively.

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human DLL3 Protein, CF Summary

• Source: Human embryonic kidney cell, HEK293-derived human DLL3 protein
  

  Human DLL3 (Ala27-Ala479) Accession # Q9NYJ7.1	HP	GGGSGGGSGGGS	HHHHHH
  N-terminus			C-terminus

• Accession #: Q9NYJ7.1
• N-terminal Sequence: Ala27
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 52-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DLL3 Protein, CF

• delta (Drosophila)-like 3
• Delta3
• delta-like 3 (Drosophila)
• delta-like protein 3
• DLL3
• Drosophila Delta homolog 3
• Pudgy
• SCDO1
• SCDO1delta3

Background

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands (1). Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain (2). Within the ECD, human DLL3 shares 86% aa sequence identity with mouse and rat DLL3. DLL3 is known as a divergent DSL ligand since it does not function in similar manner as the rest of DSL ligands. It does not activate Notch signaling through trans-activation, but autonomously attenuates signaling induced by other DSL ligands (3). A loss-of-function mutation of DLL3 is linked to axial skeletal defects in the spondylocostal dysplasia, which is linked to abnormal Notch signaling (4). DL-3 promotes proliferation and inhibits apoptosis of cancer cells. The proliferative effect mediated by DLL3 is thought to be due to increased Akt phosphorylation in cancer cells (5).

1. Dunwoodie, S. L. et al. (1997) Development 124:3065.
2. Bulman, M. P. et al. (2000) Nat. Genet. 24:438.
3. Ladi, E. et al. (2005) J. Cell Biol. 170:983.
4. Dunwoodie, S.L. et al. (2002) Development 129:1795.
5. Deng S.M. et al. (2017) Biochem. Biophys. Res. Commun. 483:488.

Publications for DLL3 (9749-DL)(2)

Publications using 9749-DL

• F Zhao, J He, J Tang, N Cui, Y Shi, Z Li, S Liu, Y Wang, M Ma, C Zhao, L Luo, L Li Brain milieu induces early microglial maturation through the BAX-Notch axis Nature Communications, 2022-10-17;13(1):6117. 2022-10-17 [PMID: 36253375] (Bioassay, Mouse)
• S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020-08-19;0(0):. 2020-08-19 [PMID: 32820046] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • Q9NYJ7.1()