Recombinant Human CSMD3 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human CSMD3 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CSMD3 protein
  Ala2218-Ser3306, with a C-terminal 6-His tag
• Accession #: Q7Z407-1
• N-terminal Sequence: Ala2218 & Tyr2219
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 119 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-147 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CSMD3 Protein, CF

• CSMD3
• CSMD-3
• CUB and sushi domain-containing protein 3
• CUB and Sushi multiple domains 3
• KIAA1894
• KIAA1894CUB and sushi multiple domains protein 3

Background

CSMD3 is a new member of CSMD (CUB and sushi multiple domains) gene family. The CSMD3 gene codes a protein of 406 kDa consisting of 3707 amino acids (aa). The CSMD3 molecule is a transmembrane protein composed of a long N-terminal polypeptide with CUB and sushi multiple domains exposing outside the cell, a single transmembrane domain, and a C-terminal domain of 55 aa located in the cytoplasm (1). CSMD3 is expressed at low levels in many tissues with highest expression in fetal and adult brains (2). Little is known about the potential function of CSMS3. CUB and sushi domains are thought to be sites of protein‑protein or protein-ligand interactions, suggesting that CSMD3 protein is either a transmembrane receptor or adhesion molecule (3, 4). The other two members of CSMD family are CSMD1 and CSMD2. CSMD1 was reproted as a candidate suppressor of head and neck squamous cell carcinomas (5). It is suspected that CSMD3 could also function as a tumor suppressor.

1. Shimizu, A. et al. (2003) Biochem. Biophy. Res. Commun. 309:143.
2. Lau, W.L. and S.B. Scholnick (2003) Genomics 82:412.
3. Bork, P. and G. Beckmann (1993) J. Mol. Biol. 231:539.
4. Norman, D.G. et al. (1991) J. Mol. Biol. (1991) 219:717.
5. Sun, P.C. et al. (2001) Genomics 75:17.

Bioinformatics

• Uniprot:
  • Q7Z407-1()