Recombinant Human CL-K1/COLEC11 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human CL-K1/COLEC11 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.
• Source: Mouse myeloma cell line, NS0-derived human CL-K1/COLEC11 protein
  Gln26-Met271, with a C-terminal 6-His tag
• Accession #: Q9BWP8
• N-terminal Sequence: No results obtained: Gln26 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: COLEC11
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CL-K1/COLEC11 Protein, CF

• CLK1
• CL-K1
• CL-K1-I
• CL-K1-II
• CL-K1-IIa
• CL-K1-IIb
• COLEC11
• collectin kidney I
• Collectin kidney protein 1
• collectin sub-family member 11
• collectin-11
• DKFZp686N1868
• MGC3279

Background

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain an N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the blood (4, 5). Alternative splicing may generate multiple isoforms with deletions or substitutions in the N-terminal and collagen-like domains (6). CL-K1 is widely expressed, notably in renal proximal tubules, bronchial glands, and gastrointestinal mucosa (5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature human CL-K1 shares 93% amino acid sequence identity with mouse and rat CL-K1. Within the CRD, human CL-K1 shares 55% aa sequence identity with CL-L1 and 26% - 33% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

1. Gupta, G. and A. Surolia, 2007, Bioessays 29:452.
2. van de Wetering, J.K. et al., 2004, Eur. J. Biochem. 271:1229.
3. Holmskov, U. et al., 2003, Annu. Rev. Immunol. 21:547.
4. Keshi, H. et al., 2006, Microbiol. Immunol. 50:1001.
5. Motomura, W. et al., 2008, J. Histochem. Cytochem. 56:243.
6. SwissProt # Q9BWP8.

Publications for CL-K1/COLEC11 (5025-CL)(1)

Publication using 5025-CL

• Rooryck C, Diaz-Font A, Osborn DP Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome. Nat. Genet., 2011-01-23;43(3):197-203. 2011-01-23 [PMID: 21258343] (Bioassay, In Vivo, Human, Zebrafish)

Bioinformatics

• Gene Symbol: COLEC11
• Uniprot:
  • Q9BWP8(Human)