Recombinant Human CEACAM-6/CD66c Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CEACAM-6/CD66c Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 10⁵ cells/well are added to CEACAM-6 coated plates (10 µg/mL, 100 µL/well), 35‑60% of the cells will adhere after 20 minutes at 37° C.
• Source: Mouse myeloma cell line, NS0-derived human CEACAM-6/CD66c protein
  Lys35-Gly320, with a C-terminal 6-His tag
• Accession #: Q53XP7
• N-terminal Sequence: Lys35
• Protein/Peptide Type: Recombinant Proteins
• Gene: CEACAM6
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 32.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 57-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CEACAM-6/CD66c Protein, CF

• carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific crossreacting antigen)
• carcinoembryonic antigen-related cell adhesion molecule 6
• CD66c antigen
• CD66c
• CEACAM6
• CEACAM-6
• CEAL
• NCA
• NCACEAL
• Non-specific crossreacting antigen
• Normal cross-reacting antigen

Background

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific crossreacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1 - 4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and GPI-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2 - 4). It shows considerable glycosylation, including (sialyl) Lewis^X, which mediates binding to E-selectin, galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs 1, 5 (CEA) and 8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments alpha _v beta ₃ and beta ₂ integrin-mediated adhesion, apparently by src and caveolin-mediated inside-out integrin activation(8, 13, 14).

1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
2. Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
3. Barnett, T. et al. (1988) Genomics 3:59.
4. Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
5. Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
6. Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
7. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
8. Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
9. Ilantzis, C. et al. (2002) Neoplasia 4:151.
10. Kalina, T. et al. (2005) BMC Cancer 5:38.
11. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
12. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
13. Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
14. Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.

Publications for CEACAM6/CD66c (3934-CM)(2)

Publications using 3934-CM

• Kim E, Cha Y, Jeong S, Chang Y Overexpression of CEACAM6 activates Src-FAK signaling and inhibits anoikis, through homophilic interactions in lung adenocarcinomas. Transl Oncol, 2022-03-28;20(0):101402. 2022-03-28 [PMID: 35358791] (Bioassay, Human)
• SK Rahman, MA Ansari, P Gaur, I Ahmad, C Chakravart, DK Verma, A Sharma, S Chhibber, N Nehal, D Wirth, SK Lal The Immunomodulatory CEA Cell Adhesion Molecule 6 (CEACAM6/CD66c) Is a Protein Receptor for the Influenza a Virus Viruses, 2021-04-21;13(5):. 2021-04-21 [PMID: 33919410] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: CEACAM6
• Uniprot:
  • Q53XP7()