Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF

1 μg/lane of Recombinant Human CD2F/SLAMF9 Fc Chimera (Catalog # 8235-CD) wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions andvisualized by silver staining, showing R bands at 60-70 kDa and NR bandsat 150-160 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.7-3.5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD2F-10/SLAMF9 protein
  

  Human CD2F-10/SLAMF9 (Arg19-Ser210) Accession # Q96A28	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q96A28
• N-terminal Sequence: Arg19 & Val33
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SLAMF9
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 48 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF

• CD2 family member 10
• CD2F10
• CD2F-10
• CD2F-10MGC88194
• CD84 homolog 1
• CD84H1
• CD84-H1
• CD84-H1cluster of differentiation 2 antigen family member 10
• cluster of differentiation 84 homolog 1
• SF2001
• signaling lymphocytic activation molecule family member 2001
• signaling lymphocytic activation molecule family member 9
• SLAM family member 9
• SLAMF9

Background

The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F-10 protein, also known as SF2001 and CD85-H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F-10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co-receptors for lymphocyte activation and/or adhesion (1). Mature human CD2F-10 is a type I transmembrane glycoprotein that consists of a 218 amino acid (aa) extracellular domain (ECD) with one Ig-like C2-set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2-4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F-10 does not contain consensus immunoreceptor tyrosine-based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT-2 (1). Within aa 19-210, human CD2F-10 shares 57% and 59% aa sequence identity with mouse and rat CD2F-10, respectively. Alternative splicing of human CD2F-10 generates an isoform that lacks the Ig-like C2-set domain. CD2F-10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F-10 inhibits the secretion of IL-2 from activated human T cells.

1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
2. Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
3. Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
4. Fraser, C.C. et al. (2002) Immunogenetics 53:843.

Publications for CD2F-10/SLAMF9 (8235-CD)(1)

Publication using 8235-CD

• The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation Nat Commun, 2016-05-05;7(0):11473. 2016-05-05 [PMID: 27145901] (Human)

Bioinformatics

• Gene Symbol: SLAMF9
• Uniprot:
  • Q96A28()