Recombinant Human C1qL3 Protein, CF

When Recombinant Human C1qL3 (Catalog # 9115-TN) is coated at 2 µg/mL, Recombinant Human BAI2 Fc Chimera binds with a typical ED50 of25-150 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human C1qL3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.
• Source: E. coli-derived human C1qL3 protein
  Ser122 - Asp255, with an N-terminal Met and 6-His tag
• Accession #: Q5VWW1
• N-terminal Sequence: Met
• Structure / Form: Noncovalently-linked homotrimer.
• Protein/Peptide Type: Recombinant Proteins
• Gene: C1QL3
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 14 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human C1qL3 Protein, CF

• C1ql
• C1qL3
• C1q-like 3
• C1qTNF13
• Complement C1q-Like Protein 3
• complement component 1, q subcomponent-like 3
• CTRP13
• K100

Background

C1qTNF proteins constitute a highly conserved family of Acrp30/Adiponectin paralogs that share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a C-terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. C1qTNF3, also known as CORS26, is predominantly expressed in cartilage and is induced in mature adipocytes. C1qTNF10, also known as C1qL2, is a protein that shows 28% - 30% sequence identity with C1q subunits A, B, and C. It is predicted to contain one collagen-like and C1q domain. Mouse and human C1qTNF10 share over 90% amino acid sequence identity.

Bioinformatics

• Gene Symbol: C1QL3
• Uniprot:
  • Q5VWW1()