Recombinant Human BCAM Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BCAM Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human BCAM protein
  

  Human BCAM (Glu32 - Ala547) Accession # CAA58449	DIEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: CAA58449
• N-terminal Sequence: Glu32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: BCAM
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 83.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BCAM Fc Chimera Protein, CF

• antigen identified by monoclonal F8
• Auberger B antigen
• basal cell adhesion molecule (Lu and Au blood groups)
• basal cell adhesion molecule (Lutheran blood group)
• basal cell adhesion molecule
• B-CAM cell surface glycoprotein
• BCAM
• B-cell adhesion molecule
• CD239 antigen
• CD239
• F8/G253 antigen
• glycoprotein 95kDa
• LUAU
• Lutheran antigen
• Lutheran blood group (Auberger b antigen included)
• Lutheran blood group glycoprotein
• MSK19

Background

Human Basal Cell Adhesion Molecule (BCAM), also known as CD239, is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule (Lu). BCAM lacks a 40 amino acid (aa) SH3-containing segment that is present in the cytoplasmic domain of Lutheran (1). The two isoforms are expressed as 85 kDa and 78 kDa glycoproteins (2 - 4). A polymorphism at position 77 within the extracellular domain (ECD) of human BCAM is the basis for the difference between the Lu^a and Lu^b Lutheran blood groups (5). The ECD of human BCAM contains two Ig-like V-type domains and three Ig-like C2-type domains (5, 6). It shares 73% aa sequence identity with the ECDs of mouse and rat BCAM. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation (3, 7 - 9). On polarized epithelium, the Lutheran isoform is restricted to the basolateral membrane, while the short isoform is also found on the apical face (2). In the superficial layer of stratified epithelium, however, it shows a nonpolarized distribution (3). BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle (3, 8, 10, 11). BCAM is upregulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas (3, 7, 9, 10). It cooperates with Integrins beta 1 and alpha V beta 3 as an adhesion receptor for Laminins which contain the alpha 5 chain (4, 12). Mouse BCAM binds to both human and mouse Laminin, whereas human BCAM binds to human but not to mouse Laminin (13). In contrast to mouse, human BCAM is additionally expressed on erythrocytes and is upregulated on these cells in sickle cell disease and polycythemia vera (8, 14, 15). It mediates increased binding of erythrocytes to Laminin and promotes the formation of erythrocyte-monocyte aggregates (8, 14 - 18). The Lutheran isoform is aberrantly phosphorylated in erythroid disorders and can enhance Laminin-mediated adhesion of erythrocytes to vascular endothelial cells (15, 18).

1. Rahuel, C. et al. (1996) Blood 88:1865.
2. El Nemer, W. et al. (1999) J. Biol. Chem. 274:31903.
3. Garin-Chesa, P. et al. (1994) Int. J. Oncol. 5:1261.
4. Vainionpaa, N. et al. (2006) Am. J. Physiol. Cell. Physiol. 290:C764.
5. El Nemer, W. et al. (1997) Blood 89:4608.
6. Campbell, I.G. et al. (1994) Cancer Res. 54:5761.
7. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
8. Rahuel, C. et al. (2008) Am. J. Physiol. Renal Physiol. 294:F393.
9. Rettig, W.J. et al. (1986) Cancer Res. 46:6406.
10. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. 85:3110.
11. Nishimune, H. et al. (2008) J. Cell Biol. 182:1201.
12. Kikkawa, Y. et al. (2007) J. Biol. Chem. 282:14853.
13. Rahuel, C. et al. (1999) Immunogenetics 50:271.
14. Zen, Q. et al. (1999) J. Biol. Chem. 274:728.
15. Wautier, M.-P. et al. (2007) Blood 110:894.
16. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
17. Chaar, V. et al. (2010) Haematologica 95:1841.
18. Gauthier, E. et al. (2009) Br. J. Haematol. 148:456.

Publications for BCAM/CD239 (148-BC)(2)

Publications using 148-BC

• Parimala Sonika Godavarthy, Christina B. Walter, Claudia Lengerke, Gerd Klein The Laminin Receptors Basal Cell Adhesion Molecule/Lutheran and Integrin alpha 7 beta 1 on Human Hematopoietic Stem Cells Frontiers in Cell and Developmental Biology 2021-10-22 [PMID: 34746117] (Bioassay, Human)
• Huang J, Filipe A, Rahuel C, Bonnin P, Mesnard L, Guerin C, Wang Y, Le Van Kim C, Colin Y, Tharaux P Lutheran/basal cell adhesion molecule accelerates progression of crescentic glomerulonephritis in mice. Kidney Int, 2014-01-15;85(5):1123-36. 2014-01-15 [PMID: 24429403] (Adhesion Assay, Human)

Bioinformatics

• Gene Symbol: BCAM
• Uniprot:
  • CAA58449()