Recombinant Human Angiopoietin-like 5 Protein

Product Discontinued

Recombinant Human Angiopoietin-like 5 Protein Summary

• Details of Functionality: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 70-280 ng/mL in the presence of a cross‑linking Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
• Source: Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 5/ANGPTL5 protein
  Met1-Lys388 with a C-terminal 6-His tag
• Accession #: AAH49170
• N-terminal Sequence: Val23 & Asn26
• Protein/Peptide Type: Recombinant Proteins
• Gene: ANGPTL5
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 42.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 5 Protein

• Angiopoietin like Protein 5
• angiopoietin-like 5
• Angiopoietin-like Protein 5
• angiopoietin-related protein 5
• ANGPTL5

Background

Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled‑coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).

1. Oike, Y. et al. (2009) Circ. J. 73:2192.
2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
3. Zeng, L. et al. (2003) J. Hum. Genet. 48:159.
4. Romeo, S. et al. (2009) J. Clin. Invest. 119:70.
5. Liu, D.J. and S.M. Leal (2010) PloS Genet. 6:e1001156.
6. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
7. Zhang, C.C. et al. (2008) Blood 111:3415.
8. Khoury, M. et al. (2010) Stem Cells Dev. Dec 8 epub.

Bioinformatics

• Gene Symbol: ANGPTL5
• Uniprot:
  • AAH49170()