Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human alpha ₂‑Macroglobulin-like1 is immobilized at 2 μg/mL (100 µL/well), the concentration of Recombinant Human LRP‑1 Cluster II Fc Chimera (Catalog # 2368-L2) that produces 50% of the optimal binding response is approximately 1‑5 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human alpha 2-Macroglobulin-like 1/A2ML1 protein
  Glu18-Glu1454, with a C-terminal 6-His tag
• Accession #: NP_653271
• N-terminal Sequence: Starts at Glu18
• Protein/Peptide Type: Recombinant Proteins
• Gene: A2ML1
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 155‑175 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF

• A2ML1
• alpha 2Macroglobulin like 1
• alpha 2-Macroglobulin-like 1
• alpha-2-macroglobulin-like 1
• alpha-2-macroglobulin-like protein 1
• C3 and PZP-like, alpha-2-macroglobulin domain containing 9
• CPAMD9

Background

alpha ₂-Macroglobulin like-1 (A2ML1) is an approximately 180 kDa secreted glycoprotein that is structurally and functionally similar to alpha ₂‑Macroglobulin (A2M) (1). Mature human A2ML1 shares 40% amino acid sequence identity with human A2M. It functions as a broad spectrum protease inhibitor with activity toward chymotrypsin, papain, thermolysin, and subtilisin A (2). Like A2M, A2ML1 binds to proteases covalently and contains a bait region (2). When the bait region is cleaved by a targeted protease, A2M undergoes a conformational change which blocks the protease’s active site and also promotes A2M clearance from the circulation (1). In contrast to the dimeric and tetrameric A2M, A2ML1 is secreted as a monomer (2, 3). A2ML1 is expressed in epidermis, testis, thymus, and placenta (2). It binds to Kallikrein 7, a protease that regulates epidermal desquamation by disrupting corneocyte adhesion (2, 4). The C‑terminal domain of A2ML1 mediates binding to LRP1 on the surface of macrophages followed by endocytosis (5). A2M has a similar receptor‑binding domain which is responsible for A2M interactions with LRP1 (6). A2ML1 is also known as the keratinocyte p170 autoantigen in the skin disease paraneoplastic pemphigus (7). During zebrafish development, an A2ML1-like protein is required for liver formation (8).

1. Sottrup-Jensen, L. (1989) J. Biol. Chem. 264:11539.
2. Galliano, M.-F. et al. (2006) J. Biol. Chem. 281:5780.
3. Gettins, P.G. et al. (1995) J. Biol. Chem. 270:14160.
4. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
5. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
6. Sottrup-Jensen, L. et al. (1986) FEBS Lett. 205:20.
7. Schepens, I. et al. (2010) PLoS ONE 5:e12250.
8. Hong, S.-K. and I.B. Dawid (2008) PLoS ONE 3:e3736.

Bioinformatics

• Gene Symbol: A2ML1
• Uniprot:
  • NP_653271(Human)