RASSF6 Antibody

Western Blot: RASSF6 Antibody [NBP1-00232] - Analysis of RASSF6 in HepG2 cell lysate (35ug protein in RIPA buffer) using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RASSF6 Antibody Summary

• Immunogen: Peptide with sequence C-SNTLKPHAKDEPDS corresponding to internal region according to NP_803876.1, NP_958834.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_803876.1; NP_958834.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RASSF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in HepG2 lysates (calculated MW of 43.4 kDa band according to NP_958834.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RASSF6 Antibody

• DKFZp686K23225
• putative RAS binding protein
• Ras association (RalGDS/AF-6) domain family 6
• Ras association (RalGDS/AF-6) domain family member 6
• ras association domain-containing protein 6

Background

RASSF6 is a member of the RAS association (RA) domain family of RAS effectors/tumor suppressors that mediate some ofthe growth inhibitory properties of RAS (see HRAS; MIM 190020) oncogenes (Eckfeld et al., 2004 (PubMed15574778)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASSF6 Antibody (NBP1-00232)(1)

Publication using NBP1-00232

• Allen NP, Donninger H, Vos MD et al. RASSF6 is a novel member of the RASSF family of tumor suppressors. Oncogene 2007-09-13 [PMID: 17404571]

Bioinformatics

• Gene Symbol: RASSF6
• Entrez:
  • 166824(Human)
• Uniprot:
  • NP_803876.1(Human)