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RASSF6 Antibody

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Western Blot: RASSF6 Antibody [NBP1-00232] - Analysis of RASSF6 in HepG2 cell lysate (35ug protein in RIPA buffer) using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RASSF6 Antibody Summary

Immunogen
Peptide with sequence C-SNTLKPHAKDEPDS corresponding to internal region according to NP_803876.1, NP_958834.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_803876.1; NP_958834.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RASSF6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in HepG2 lysates (calculated MW of 43.4 kDa band according to NP_958834.1).
Publications
Read Publication using NBP1-00232.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RASSF6 Antibody

  • DKFZp686K23225
  • putative RAS binding protein
  • Ras association (RalGDS/AF-6) domain family 6
  • Ras association (RalGDS/AF-6) domain family member 6
  • ras association domain-containing protein 6

Background

RASSF6 is a member of the RAS association (RA) domain family of RAS effectors/tumor suppressors that mediate some ofthe growth inhibitory properties of RAS (see HRAS; MIM 190020) oncogenes (Eckfeld et al., 2004 (PubMed15574778)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RASSF6 Antibody (NBP1-00232)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RASSF6
Entrez
Uniprot