RAR beta/NR1B2 Antibody

Immunohistochemistry-Paraffin: RAR beta/NR1B2 Antibody [NB110-39045] - Analysis of anti-RARB antibody with human intermediate trophoblasts at concentration 10 ug/ml.

Product Discontinued

RAR beta/NR1B2 Antibody Summary

• Immunogen: Synthetic 13 amino acid peptide from C-terminus of human RAR beta/NR1B2.
• Epitope: C-terminal region of human
• Localization: Cytoplasmic (isoform beta 4) and Nuclear (isoforms beta 1 and beta 2).
• Specificity: Human RARB. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Chicken (92%), Canine (92%), Turkey (92%), Opossum (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RARB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 21785820).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Mouse (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAR beta/NR1B2 Antibody

• beta polypeptide
• HAP
• HBV-activated protein
• NR1B2
• RAR beta
• RARB
• retinoic acid receptor, beta
• RRB2

Background

Retinoic acid receptor beta (RAR beta), a NR1 Thyroid Hormone-Like Receptor, is involved in the regulation of cell growth, differentiation, apoptosis, and carcinogenesis. RAR beta forms heterodimers with the retinoic acid X receptor (RXR) and binds to the direct repeat 1 (DR1) sequence of target DNA. A growing body of evidence supports the hypothesis that RAR beta is a tumor-suppressor gene. Altered expression of RAR beta has been linked to cancers, including those of the head and neck, lung, esophagus, breast, pancreas, and cervix. Three isoforms of RAR beta, beta 1, beta 2, and beta 4, have been documented in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAR beta/NR1B2 Antibody (NB110-39045)(1)

Publication using NB110-39045

• Fabricius EM, Kruse-Boitschenko U, Schneeweiss U et al. Model examination of chemoprevention with retinoids in squamous cell carcinomas of the head and neck region and suitable biomarkers for chemoprevention. Int J Oncol. 2011-11-01 [PMID: 21785820] (IHC-Fr, Human)

FAQs for RAR beta/NR1B2 Antibody (NB110-39045). (Showing 1 - 1 of 1 FAQ).

1. I am planning to perform IHC staining of RARB on FFPE samples. I have found the reference "J Cutan Pathol 2009;36; 1141-1145" used an antibody from your company. Would you let me know any other references using the antibody for IHC staining of RARB?
   • Unfortunately we are not aware of any other publication wherein our Retinoic Acid Receptor beta antibodies were cited for their use in IHC application. From our sales records, it looks like the researcher used catalog number NB200-323 in the publication you mentioned. Retinoic Acid Receptor beta antibody NB200-323 is our best selling product among this category and it has also been cited for its use in WB application by Yang et al. in Am J Physiol Renal Physiol. 2008 Jun;294(6):F1433-40. We have at least 7 primary antibody products to Retinoic Acid Receptor beta that have been validated for IHC-P application.. We stand behind the quality of our products and offer 100% guarantee for the applications/species mentioned on the datasheets. We are always there to help if you come up with any difficulties and yes, we provide replacements/refunds after troubleshooting a problem, if you do not get your desired outcome! Therefore, please let us know if you have any specific questions or concerns on the IHC-P application of our Retinoic Acid Receptor beta antibodies. You may contact us at technical@novusbio.com.

Bioinformatics

• Gene Symbol: RARB
• Entrez:
  • 5915(Human)