Rapsyn Antibody

Western Blot: Rapsyn Antibody [NBP1-37778] (0.5ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Rapsyn Antibody Summary

• Description: Novus Biologicals Goat Rapsyn Antibody (NBP1-37778) is a polyclonal antibody validated for use in WB and ELISA. Anti-Rapsyn Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence YQSNQTEKALQVWTK-C corresponding to N-Terminus according to NP_005046.2, NP_116034.2.
• Epitope: N-Terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005046.2; NP_116034.2).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAPSN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in human skeletal muscle lysates (calculated MW of 39.9 kDa band according to NP_116034.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rapsyn Antibody

• 43 kDa postsynaptic protein
• Acetylcholine receptor-associated 43 kDa protein
• CMS1D43 kDa receptor-associated protein of the synapse
• CMS1EMGC3597
• rapsyn
• receptor-associated protein of the synapse
• receptor-associated protein of the synapse, 43kD
• RNF205RING finger protein 205

Background

This protein belongs to a family of proteins that are receptor associated proteins of the synapse. It contains a conserved cAMP-dependent protein kinase phosphorylation site. It is believed to play some role in anchoring or stabilizing the nicotinic acetylcholine receptor at synaptic sites. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Two splice variants have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rapsyn Antibody (NBP1-37778)(1)

Publication using NBP1-37778

• Banwell BL, Ohno K, Sieb JP, Engel AG. Novel truncating RAPSN mutations causing congenital myasthenic syndrome responsive to 3,4-diaminopyridine. Neuromuscul Disord 2004-03-01 [PMID: 15036330]

Bioinformatics

• Gene Symbol: RAPSN
• Entrez:
  • 5913(Human)
• Uniprot:
  • NP_005046.2(Human)