Recombinant Human Rap2B His Protein

SDS-Page: RAP2B Protein [NBP2-23396]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Rap2B His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-183 of Human Rap2B
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMREYKVV VLGSGGVGKS ALTVQFVTGS FIEKYDPTIE DFYRKEIEVD SSPSVLEILD TAGTEQFASM RDLYIKNGQG FILVYSLVNQ QSFQDIKPMR DQIIRVKRYE RVPMILVGNK VDLEGEREVS YGEGKALAEE WSCPFMETSA KNKASVDELF AEIVRQMNYA AQPNGDEGCC SACVIL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RAP2B
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Rap2B His Protein

• MGC20484
• Rap2B
• RAP2B, member of RAS oncogene family
• Ras family small GTP binding protein RAP2B
• ras-related protein Rap-2b
• small GTP binding protein

Background

Ras-related protein Rap-2b, also known as RAP2B, belongs to a family of RAS-related genes. Unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. RAP2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway. Recombinant human RAP2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RAP2B