RAMP2 Antibody

Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF Membrane was probed with 1 µg/mL of Mouse RAMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6500) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

bEnd.3 mouse endothelioma cell line was stained with Mouse RAMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6500, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

RAMP2 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse RAMP2
  Ser45-Val159
  Accession # Q9WUP0
• Specificity: Detects mouse RAMP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) RAMP2 is observed, and less than 1% cross-reactivity with rhRAMP1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: RAMP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RAMP2 Antibody

• calcitonin receptor-like receptor activity modifying protein 2
• Calcitonin-receptor-like receptor activity-modifying protein 2
• CRLR activity-modifying protein 2
• RAMP2
• receptor (calcitonin) activity modifying protein 2
• receptor (G protein-coupled) activity modifying protein 2
• receptor activity modifying protein 2
• receptor activity-modifying protein 2
• receptor-activity-modifying protein 2

Background

RAMP2 (receptor activity modifying protein 2) is a 20 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115 aa extracellular domain (ECD) (aa 45-159) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa 100-106 are critical for AM binding. Over aa 45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAMP2
• Uniprot:
  • Q9WUP0(Mouse)