RAMP2 Antibody [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

RAMP2 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant mouse RAMP2
  Ser45-Val159
  Accession # Q9WUP0
• Specificity: Detects mouse RAMP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) RAMP2 is observed, and less than 1% cross-reactivity with rhRAMP1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RAMP2 Antibody [Alexa Fluor® 405]

• calcitonin receptor-like receptor activity modifying protein 2
• Calcitonin-receptor-like receptor activity-modifying protein 2
• CRLR activity-modifying protein 2
• RAMP2
• receptor (calcitonin) activity modifying protein 2
• receptor (G protein-coupled) activity modifying protein 2
• receptor activity modifying protein 2
• receptor activity-modifying protein 2
• receptor-activity-modifying protein 2

Background

RAMP2 (receptor activity modifying protein 2) is a 20 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115 aa extracellular domain (ECD) (aa 45-159) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa 100-106 are critical for AM binding. Over aa 45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.