RalB Antibody (4D1) - Azide and BSA Free

Western Blot: RalB Antibody (4D1) [H00005899-M04] - Analysis of RALB expression in transfected 293T cell line by RALB monoclonal antibody (M04), clone 4D1. Lane 1: RALB transfected lysatE (23.4 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: RalB Antibody (4D1) [H00005899-M04] - Analysis of monoclonal antibody to RALB on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml

Immunoprecipitation: RalB Antibody (4D1) [H00005899-M04] - Analysis of RALB transfected lysate using anti-RALB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RALB rabbit polyclonal antibody.

ELISA: RalB Antibody (4D1) [H00005899-M04] - Detection limit for recombinant GST tagged RALB is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 4D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RalB Antibody (4D1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPVEEARSKAEEWGVQYVETSAKTRANVDKVFFDLMREIRTKKMSE
• Specificity: RALB (4D1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RALB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RalB Antibody (4D1) - Azide and BSA Free

• GTP binding protein
• RalB
• RAS-like protein B
• ras-related protein Ral-B
• v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)

Background

This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RalB Antibody (H00005899-M04)(1)

Publication using H00005899-M04

• Lee BY, Jethwaney D, Schilling B et al. The mycobacterium bovis BCG phagosome proteome. Mol Cell Proteomics. 2009-10-07 [PMID: 19815536]

Bioinformatics

• Gene Symbol: RALB
• Entrez:
  • 5899(Human)
• OMIM:
  • 179551(Human)
• Uniprot:
  • AAH18163(Human)