RAE1 Antibody

Images

 
Western Blot: RAE1 Antibody [NB100-1894] - Staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RAE1 Antibody Summary

Immunogen
Peptide with sequence C-NAAEELKPRNKK corresponding to C-Terminus according to NP_003601.1, NP_001015885.1.
Specificity
NP_003601and NP_001015885 represent identical proteins.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RAE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 40 kDa band observed in lysates HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0 kDa band according to NP_003601.1). IHC: Human brain cortex shows staining of neuronal cell bodies.
Control
RAE1 Overexpression Lysate
Publications
Read Publication using NB100-1894.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAE1 Antibody

  • dJ481F12.3
  • dJ800J21.1
  • FLJ30608
  • homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer)
  • MGC117333
  • MGC126076
  • MGC126077
  • MIG14
  • migration-inducing gene 14
  • Mnrp41
  • mRNA export factor
  • mRNA export protein
  • mRNA-associated protein mrnp 41
  • mRNA-binding protein, 41-kD
  • MRNP41
  • RAE1 (RNA export 1, S.pombe) homolog
  • Rae1 protein homolog
  • RAE1 RNA export 1 homolog (S. pombe)

Background

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAE1 Antibody (NB100-1894)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol RAE1
Entrez
Uniprot