Rab1A Antibody - BSA Free

Western Blot: Rab1A Antibody [NBP2-20037] - Western Blot analysis of RAB1A expression in transfected 293T cell line by RAB1A polyclonal antibody. A: Non-transfected lysate. B: RAB1A transfected lysate. 12% SDS PAGE gel, diluted at 1:500.

Western Blot: Rab1A Antibody [NBP2-20037] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Rab1A Antibody [NBP2-20037] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (NBP2-20037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt, Po, Ca, Ch, RM, Sh, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Rab1A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Rab1A Antibody - BSA Free (NBP2-20037) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Rab1A. The exact sequence is proprietary.
• Localization: Golgi apparatus; Endoplasmic reticulum
• Predicted Species: Porcine (99%), Rhesus Macaque (100%), Chicken (100%), Canine (100%), Sheep (100%), Xenopus (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAB1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (97%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Rab1A Antibody - BSA Free

• DKFZp564B163
• member RAS oncogene family
• RAB1
• Rab1A
• RAB1A, member RAS oncogene family
• ras-related protein Rab-1A

Background

This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RAB1A
• Uniprot:
  • P62820(Human)