PYK2/FAK2 Antibody (SC06-15)

Western Blot: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of PYK2 on mouse brain lysates using anti-PYK2 antibody at 1:1000 dilution.

Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded mouse kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded human kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded human spleen tissue using anti-PYK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded human tonsil tissue using anti-PYK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded mouse brain tissue using anti-PYK2 antibody. Counter stained with hematoxylin.

Product Discontinued

PYK2/FAK2 Antibody (SC06-15) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal human PYK2/FAK2. (SwissProt: Q14289 Human; SwissProt: Q9QVP9 Mouse; SwissProt: P70600 Rat)
• Localization: Cell membrane, Nucleus, Cytoplasm, perinuclear region, focal adhesion, lamellipodium, cell cortex.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PTK2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PYK2/FAK2 Antibody (SC06-15)

• CADTK
• CADTKCAK-beta
• CAK beta
• CAKB
• Calcium-dependent tyrosine kinase
• Cell adhesion kinase beta
• EC 2.7.10
• FADK2
• FAK2
• FAK2EC 2.7.10.2
• Focal adhesion kinase 2
• FRNK
• PKB
• ppyk2
• Proline-rich tyrosine kinase 2
• protein kinase B
• protein tyrosine kinase 2 beta
• protein-tyrosine kinase 2-beta
• PTK
• PTK2B protein tyrosine kinase 2 beta
• PTK2B
• PYK2
• PYK2Related adhesion focal tyrosine kinase
• RAFTK
• RAFTKFADK 2

Background

Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK2) related protein kinase protein also known as cell adhesion kinase (CAK ), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expre ed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca+ intracellulary (1,2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for PYK2/FAK2 Antibody (NBP2-67627) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 06/06/2019

Summary

• Application: Western Blot
• Sample Tested: Lung single-cell suspension
• Species: Mouse
• Lot: HJ0629

Bioinformatics

• Gene Symbol: PTK2B